Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Submit a review Q&A (1)References (2)

Overview

  • Product name

    Anti-Galectin 1 antibody [EPR3206(2)]
    See all Galectin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3206(2)] to Galectin 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Galectin 1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • K562, Human placenta and fetal kidney lysates, Human kidney and Human skin tissues

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab138513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.

    • Tissue specificity

      Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

    • Sequence similarities

      Contains 1 galectin domain.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 14 kDa laminin-binding protein antibody
      • 14 kDa lectin antibody
      • Beta galactoside binding lectin antibody
      • Beta galactoside binding lectin L 14 I antibody
      • beta galactoside binding protein antibody
      • Beta-galactoside-binding lectin L-14-I antibody
      • DKFZp686E23103 antibody
      • Gal 1 antibody
      • Gal-1 antibody
      • GAL1 antibody
      • Galaptin antibody
      • Galbp antibody
      • Galectin antibody
      • Galectin-1 antibody
      • Galectin1 antibody
      • GBP antibody
      • HBL antibody
      • HLBP14 antibody
      • HPL antibody
      • L 14.5 antibody
      • L-14.5 antibody
      • L14 antibody
      • Lactose binding lectin 1 antibody
      • Lactose-binding lectin 1 antibody
      • Lect14 antibody
      • Lectin galactoside binding soluble 1 antibody
      • Lectin galactoside-binding soluble 1 antibody
      • LEG1_HUMAN antibody
      • LGALS 1 antibody
      • LGALS1 antibody
      • Lgals1 lectin galactose binding soluble 1 antibody
      • MAPK activating protein MP12 antibody
      • Putative MAPK activating protein MP12 antibody
      • Putative MAPK-activating protein PM12 antibody
      • S Lac lectin 1 antibody
      • S-Lac lectin 1 antibody
      see all

    Images

    • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 15 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab138513 was shown to specifically react with Galectin 1 in wild-type HAP1 cells as signal was lost in LGALS1 (Galectin 1) knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. ab138513 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human placenta lysate
      Lane 3 : Fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 15 kDa
      Observed band size: 14 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Flow cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) labeling Galectin 1 with ab138513 at 1/100 dilution (red).  ab150077 at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde
      and permeabilised with 90% methanol. 

      Isotype control - Rabbit monoclonal IgG, ab172730 (Black)
      Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue)

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Normal Human Stomach tissue using ab138513 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Normal Human Spleen tissue using ab138513 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab138513 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Normal Human Heart tissue using ab138513 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

      Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab138513 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Gao Y  et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). Read more (PubMed: 29549328) »
    • Fan SM  et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 167:121-131 (2018). WB, IHC-P ; Mouse . Read more (PubMed: 29567388) »
    See all 2 Publications for this product

    Publishing research using ab138513? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Please tell me what is the best diluent used for the western blotting ? I should prepare the antibody in milk or BSA, to maintain it stability.

    Read More
    Answer


    We suggest diluting ab138513 1/1000 in 5% BSA/TBST immediately prior to use in western blot.

    Read More

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