Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Galectin 1 antibody [EPR3206(2)]
See all Galectin 1 primary antibodies -
DescriptionRabbit monoclonal [EPR3206(2)] to Galectin 1
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Galectin 1 aa 50-150. The exact sequence is proprietary.
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Positive control
- K562, Human placenta and fetal kidney lysates, Human kidney and Human skin tissues
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3206(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab138513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | 1/10 - 1/100. |
Target
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FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
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Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
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Sequence similaritiesContains 1 galectin domain.
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Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P09382 Human
- SwissProt: P16045 Mouse
- SwissProt: P11762 Rat
- Unigene: 445351 Human
see all -
Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
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![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/ab138513-306624-ab138513KOWBJRIZ.jpg)
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 15 kDaLanes 1 - 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab138513 was shown to specifically react with Galectin 1 in wild-type HAP1 cells as signal was lost in LGALS1 (Galectin 1) knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. ab138513 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-1.jpg)
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human placenta lysate
Lane 3 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250. -
![Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/ab138513-342270-ab138513.jpg)
Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Flow cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) labeling Galectin 1 with ab138513 at 1/100 dilution (red). ab150077 at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde
and permeabilised with 90% methanol.Isotype control - Rabbit monoclonal IgG, ab172730 (Black)
Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Normal Human Stomach tissue using ab138513 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Normal Human Spleen tissue using ab138513 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab138513 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Normal Human Heart tissue using ab138513 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)](https://www.abcam.com/ps/products/138/ab138513/Images/Galectin-1-Primary-antibodies-ab138513-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab138513 showing +ve staining.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Gao Y et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). Read more (PubMed: 29549328) »
- Fan SM et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 167:121-131 (2018). WB, IHC-P ; Mouse . Read more (PubMed: 29567388) »
