Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Submit a review Q&A (1)References (4)
Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
  • Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3206(2)] to Galectin 1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Galectin 1 antibody [EPR3206(2)]
    See all Galectin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3206(2)] to Galectin 1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Galectin 1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate, mouse heart and rat heart tissue lysate IHC: human kidney tissue ICC/IF: K-562 (Human chronic myelogenous leukemia lymphoblast)

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/50.
Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/50.

Target

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.

  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

  • Sequence similarities

    Contains 1 galectin domain.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Flow Cytometry - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Galectin 1 with Purified ab138513 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/10000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 15 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Galectin 1 with Purified ab138513 at 1/50 dilution (5.4 µg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Galectin 1 with Purified ab138513 at 1/16000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution (Unpurified)

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : LGALS1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab138513 was shown to react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266850 (knockout cell lysate ab256974) was used. Wild-type HEK-293T and LGALS1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : LGALS1 (Galectin 1) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 15 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab138513 was shown to specifically react with Galectin 1 in wild-type HAP1 cells as signal was lost in LGALS1 (Galectin 1) knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. ab138513 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

References (4)

Publishing research using ab138513? Please let us know so that we can cite the reference in this datasheet.

ab138513 has been referenced in 4 publications.

  • Leung Z  et al. Galectin-1 promotes hepatocellular carcinoma and the combined therapeutic effect of OTX008 galectin-1 inhibitor and sorafenib in tumor cells. J Exp Clin Cancer Res 38:423 (2019). PubMed: 31640796
  • Zhang PF  et al. SNHG22 overexpression indicates poor prognosis and induces chemotherapy resistance via the miR-2467/Gal-1 signaling pathway in epithelial ovarian carcinoma. Aging (Albany NY) 11:8204-8216 (2019). PubMed: 31581131
  • Gao Y  et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). PubMed: 29549328
  • Fan SM  et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 167:121-131 (2018). WB, IHC-P ; Mouse . PubMed: 29567388

Customer reviews and Q&As

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Question

Please tell me what is the best diluent used for the western blotting ? I should prepare the antibody in milk or BSA, to maintain it stability.

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Answer


We suggest diluting ab138513 1/1000 in 5% BSA/TBST immediately prior to use in western blot.

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