Key features and details
- Mouse monoclonal [GAL1/1831] to Galectin 1 - BSA and Azide free
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Galectin 1 antibody [GAL1/1831] - BSA and Azide free
See all Galectin 1 primary antibodies
DescriptionMouse monoclonal [GAL1/1831] to Galectin 1 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, Protein Arraymore details
Species reactivityReacts with: Human
Recombinant fragment within Human Galectin 1 aa 12-108. The exact sequence is proprietary.
Database link: P09382
- Human prostate carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab218906 was purified from Bioreactor Concentrate by Protein A/G.
Light chain typelambda
Our Abpromise guarantee covers the use of ab218906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT).
|Protein Array||Use at an assay dependent concentration.|
FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
This data was produced with ab218446, the same antibody in a different formulation with BSA and Azide.
ab218446 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Galectin 1 with ab218906 at 1 µg/ml.
All lanes : Anti-Galectin 1 antibody [GAL1/1831] - BSA and Azide free (ab218906) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293 cell lysate
Predicted band size: 15 kDa
ab218906 has not yet been referenced specifically in any publications.