Recombinant Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
![Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-301211-anti-galectin-1-antibody-sp247-c-terminal-western-blot.jpg "Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP247] to Galectin 1 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Galectin 1 antibody [SP247] - C-terminal
See all Galectin 1 primary antibodies -
Description
Rabbit monoclonal [SP247] to Galectin 1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 cell lysate. IHC-P: Human placenta tissue. Flow Cyt (intra): HL-60 cells. IHC-Fr: Frozen human placenta and prostate carcinoma tissue sections
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
Primary antibody incubation for 30 minutes at 4°C. |
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| WB |
1/100. Predicted molecular weight: 15 kDa.
Primary antibody incubation for 1 hour at room temperature. |
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| IHC-P |
1/100.
Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
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| IHC-Fr |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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Flow Cyt (Intra)
1/100. Primary antibody incubation for 30 minutes at 4°C. |
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WB
1/100. Predicted molecular weight: 15 kDa. Primary antibody incubation for 1 hour at room temperature. |
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IHC-P
1/100. Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
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IHC-Fr
Use a concentration of 10 µg/ml. |
Target
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 326598 Cow
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 414915 Pig
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P11116 Cow
- SwissProt: P09382 Human
see all -
Alternative names
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
Images
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Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842) at 1/100 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Predicted band size: 15 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-301210-anti-galectin-1-antibody-sp247-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)Formalin-fixed, paraffin-embedded human placenta tissue stained for Galectin 1 using ab192842 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-5-antigalectin1sp247immunohistochemistryfrozenplacentahuman.jpg)
Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)IHC image of Galectin 1 staining in a section of frozen normal human placenta* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
![Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-6-antigalectin1sp247immunohistochemistryfrozenprostatecarcinomahuman.jpg)
Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)IHC image of Galectin 1 staining in a section of frozen normal human prostate carcinoma* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
![Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-301209-anti-galectin-1-antibody-sp247-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)Intracellular flow cytometric analysis ofHL-60 (Human promyelocytic leukemia cell line) cells labeling Galectin 1 with ab192842 at 1/100 dilution (green) compared to a negative control of rabbit IgG (blue).
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![Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)](https://www.abcam.com/ps/products/192/ab192842/Images/ab192842-4-benefits-of-recombinant-antibodies.png)
Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab192842 has not yet been referenced specifically in any publications.