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    galectin-1-antibody-sp247-c-terminal-ab192842.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

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Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
  • Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
  • Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP247] to Galectin 1 - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Recombinant human Galectin 1 protein (ab50237)
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Anti-galectin 9/Gal-9 antibody (ab69630)

View more associated products

Overview

  • Product name

    Anti-Galectin 1 antibody [SP247] - C-terminal
    See all Galectin 1 primary antibodies
  • Description

    Rabbit monoclonal [SP247] to Galectin 1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 cell lysate. IHC-P: Human placenta tissue. Flow Cyt (intra): HL-60 cells. IHC-Fr: Frozen human placenta and prostate carcinoma tissue sections
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP247
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • Anti-Galectin 1 antibody [SP247] - C-terminal, prediluted (ab228184)
    • Anti-Galectin 1 antibody [SP247] - BSA and Azide free (ab240972)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human Galectin 1 protein (ab50237)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.

Primary antibody incubation for 30 minutes at 4°C.

WB
1/100. Predicted molecular weight: 15 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P
1/100.

Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

IHC-Fr
Use a concentration of 10 µg/ml.
Notes
Flow Cyt (Intra)
1/100.

Primary antibody incubation for 30 minutes at 4°C.

WB
1/100. Predicted molecular weight: 15 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P
1/100.

Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

IHC-Fr
Use a concentration of 10 µg/ml.

Target

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P09382 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 326598 Cow
    • Entrez Gene: 3956 Human
    • Entrez Gene: 16852 Mouse
    • Entrez Gene: 414915 Pig
    • Entrez Gene: 56646 Rat
    • Omim: 150570 Human
    • SwissProt: P11116 Cow
    • SwissProt: P09382 Human
    • SwissProt: P16045 Mouse
    • SwissProt: Q49I35 Pig
    • SwissProt: P11762 Rat
    • Unigene: 445351 Human
    • Unigene: 43831 Mouse
    • Unigene: 57 Rat
    see all
  • Alternative names

    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842) at 1/100 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Galectin 1 using ab192842 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    IHC image of Galectin 1 staining in a section of frozen normal human placenta* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    IHC image of Galectin 1 staining in a section of frozen normal human prostate carcinoma* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    Intracellular flow cytometric analysis ofHL-60 (Human promyelocytic leukemia cell line) cells labeling Galectin 1 with ab192842 at 1/100 dilution (green) compared to a negative control of rabbit IgG (blue). 

     

     

  • Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)
    Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab192842? Please let us know so that we can cite the reference in this datasheet.

ab192842 has not yet been referenced specifically in any publications.

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