Recombinant
RabMAb

Recombinant Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

Rabbit recombinant monoclonal Galectin 1 antibody [SP247]. Validated in WB, IHC, Flow Cyt and tested in Human.

Overview

  • Product name

    Anti-Galectin 1 antibody [SP247] - C-terminal
    See all Galectin 1 primary antibodies
  • Description

    Rabbit monoclonal [SP247] to Galectin 1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide within Human Galectin 1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P09382

  • Positive control

    • WB: NIH/3T3 cell lysate. IHC-P: Human placenta tissue. Flow Cyt: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab192842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Primary antibody incubation for 30 minutes at 4°C.

WB 1/100. Predicted molecular weight: 15 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P 1/100.

Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Images

  • Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842) at 1/100 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 15 kDa

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for Galectin 1 using ab192842 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HL-60 (Human promyelocytic leukemia cell line) cells labeling Galectin 1 with ab192842 at 1/100 dilution (green) compared to a negative control of rabbit IgG (blue).

References

ab192842 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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