Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP247] to Galectin 1 - C-terminal, prediluted
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-Galectin 1 antibody [SP247] - C-terminal, prediluted
See all Galectin 1 primary antibodies
DescriptionRabbit monoclonal [SP247] to Galectin 1 - C-terminal, prediluted
Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide within Human Galectin 1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P09382
- IHC-P: Human placenta. ICC/IF: U2OS cells Flow Cyt: U2OS cells
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab228184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Ready to use.
Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Primary antibody incubation for 10 minutes at room temperature.
FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
Immunocytochemistry/ Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell) cells labeling Galectin 1 with purified ab228184 at 1/25 (3.68µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow cytometry analysis of U2OS (human bone osteosarcoma epithelial cell) labeling Galectin 1 with purified ab228184 at 1/200 dilution (0.46 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
Formalin-fixed, paraffin-embedded human placenta tissue stained for Galectin 1 using ab228184 in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228184 has not yet been referenced specifically in any publications.