Overview

  • Product name

  • Description

    Rabbit polyclonal to Galectin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Full length protein (proprietary-tag) corresponding to Human Galectin 2 aa 1-132 (N terminal).
    Sequence:

    MTGELEVKNM DMKPGSTLKI TGSIADGTDG FVINLGQGTD KLNLHFNPRF SESTIVCNSL DGSNWGQEQR EDHLCFSPGS EVKFTVTFES DKFKVKLPDG HELTFPNRLG HSHLSYLSVR GGFNMSSFKL KE


    Database link: P05162

  • Positive control

    • Human liver cancer tissue lysate, Human leiomyosarcoma tissue lysate; Human brain cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol

    without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/200 - 1/1000. Predicted molecular weight: 15 kDa.

Target

Images

  • Immunohistochemical analysis of paraffin embedded Human brain cancer tissue labeling Galectin 2 with ab182628 at 1/13 (left). The right shows the same analysis of tissue treated with the fusion protein. (Original magnification: ×200).

  • All lanes : Anti-Galectin 2 antibody (ab182628) at 1/250 dilution

    Lane 1 : Human liver cancer tissue lysate
    Lane 2 : Human leiomyosarcoma tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa


    Exposure time: 10 seconds


    Gel: 15%+12%SDS-PAGE

References

ab182628 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NMU)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
NMU
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Dec 15 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up