Key features and details
- Mouse monoclonal [5C21] to Galectin 3
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Galectin 3 antibody [5C21]
See all Galectin 3 primary antibodies
DescriptionMouse monoclonal [5C21] to Galectin 3
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Galectin 3.
Database link: P17931
- WB: Mouse brain lysate; rat brain, liver, kidney, lung. HEK-293 cell lysate. ICC: Embryonic rat (E20) cortical neuron-glial cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Tissue Culture supernatant.
Our Abpromise guarantee covers the use of ab278071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 26 kDa.|
FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
- Information by UniProt
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
Cortical neuron-glial cell culture from E20 rat stained for galectin 3 (red) using ab278071 at 1/1000 dilution in immunocytochemistry. A chicken polyclonal antibody to MAP2 (green) was used at 1/10000 dilution. The blue is Hoechst staining of nuclear DNA.
Lanes 2-8 : Anti-Galectin 3 antibody [5C21] (ab278071) at 1/2000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain
Lane 4 : Rat liver
Lane 5 : Rat heart
Lane 6 : Rat kidney
Lane 7 : Rat lung
Lane 8 : HEK-293 cell lysate
Predicted band size: 26 kDa
The band at about 30kDa mark corresponds to the galectin 3 protein. The same blot was simultaneously probed with chicken polyclonal to HSP60 (red), at 1/20000 dilution which reveals HSP60 protein in all preparations.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab278071 has not yet been referenced specifically in any publications.