Overview

  • Product name

  • Description

    Rabbit polyclonal to Galectin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Galectin 3 (internal sequence). The exact sequence is proprietary.
    Database link: P17931

  • Positive control

    • WB: HeLa cell extract; A549, H1299, HCT 116 and MCF7 whole cell lysates; Galectin 3 transfected HEK-293T cell lysate; rat colon lysate. ICC/IF: MCF7 cells. IHC-P: HBL435 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificity

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Images

  • All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Galectin 3 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa

  • Paraffin-embedded HBL435 xenograft tissue stained for Galectin 3 using ab227249 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Galectin 3 antibody (ab227249) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : Galectin 3 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

  • Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Galectin 3 (green) using ab227249 at 1/1000 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Galectin 3 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE gel-

  • Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution + Rat colon lysate at 50 µg

    Predicted band size: 26 kDa



    12 % SDS-PAGE gel.

References

ab227249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph9
Permeabilization
No
Specification
Kidney
Blocking step
(agent) for 4 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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