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    galectin-3-antibody-ab31707.pdf

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Neuroscience Cell Adhesion Proteins Nuclear Proteins
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Validated using a knockout cell line

Anti-Galectin 3 antibody (ab31707)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (9)

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Western blot - Anti-Galectin 3 antibody (ab31707)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab31707)
  • Western blot - Anti-Galectin 3 antibody (ab31707)

Key features and details

  • Rabbit polyclonal to Galectin 3
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant human Galectin 3 protein (Active) (ab50236)

View more associated products

Overview

  • Product name

    Anti-Galectin 3 antibody
    See all Galectin 3 primary antibodies
  • Description

    Rabbit polyclonal to Galectin 3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Galectin 3 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab31706)

  • Positive control

    • Recombinant human Galectin 3 protein (ab50236) can be used as a positive control in WB. HeLa (Human epithelial carcinoma cell line) Whole Cell and Nuclear Lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Nuclear Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab29545)
    • Recombinant human Galectin 3 protein (Active) (ab50236)
    • Recombinant human Galectin 3 protein (ab78583)
  • Recombinant Protein

    • Recombinant human Galectin 3 protein (Active) (ab50236)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Dog
Pig
Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 µg/ml.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificity

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3958 Human
    • Omim: 153619 Human
    • SwissProt: P38486 Dog
    • SwissProt: P17931 Human
    • Unigene: 531081 Human
    • Alternative names

      • 35 kDa lectin antibody
      • Carbohydrate binding protein 35 antibody
      • Carbohydrate-binding protein 35 antibody
      • CBP 35 antibody
      • CBP35 antibody
      • Gal-3 antibody
      • GAL3 antibody
      • Galactose-specific lectin 3 antibody
      • Galactoside-binding protein antibody
      • GALBP antibody
      • Galectin 3 internal gene,included antibody
      • Galectin-3 antibody
      • Galectin3 antibody
      • GALIG antibody
      • GBP antibody
      • IgE binding protein antibody
      • IgE-binding protein antibody
      • L 31 antibody
      • L 34 antibody
      • L-31 antibody
      • L-34 galactoside-binding lectin antibody
      • L31 antibody
      • Laminin-binding protein antibody
      • Lectin L-29 antibody
      • Lectin, galactose binding, soluble 3 antibody
      • LEG3_HUMAN antibody
      • LGALS2 antibody
      • LGALS3 antibody
      • MAC 2 antigen antibody
      • Mac-2 antibody
      • Mac-2 antigen antibody
      • MAC2 antibody
      • Macrophage galactose-specific lectin antibody
      • MGC105387 antibody
      see all

    Images

    • Western blot - Anti-Galectin 3 antibody (ab31707)
      Western blot - Anti-Galectin 3 antibody (ab31707)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)              
      Lane 2: Galectin 3  knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab31707 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab31707 was shown to specifically recognize Galectin 3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Galectin 3 knockout samples were examined. Wild-type and Galectin 3 knockout samples were subjected to SDS-PAGE.  Ab31707 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab31707)
      Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab31707)

      ICC/IF image of ab31707 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31707, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • Western blot - Anti-Galectin 3 antibody (ab31707)
      Western blot - Anti-Galectin 3 antibody (ab31707)
      All lanes : Anti-Galectin 3 antibody (ab31707) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 26 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?



      We also see a very faint band at 52 kDa in the HeLa Whole cell lysate, we are uncertain as to the identity of this band.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab31707? Please let us know so that we can cite the reference in this datasheet.

    ab31707 has been referenced in 9 publications.

    • Koerver L  et al. The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage. EMBO Rep 20:e48014 (2019). PubMed: 31432621
    • Coppin L  et al. Galectin-3 is a non-classic RNA binding protein that stabilizes the mucin MUC4 mRNA in the cytoplasm of cancer cells. Sci Rep 7:43927 (2017). PubMed: 28262838
    • Zheng J  et al. Galectin-3 induced by hypoxia promotes cell migration in thyroid cancer cells. Oncotarget 8:101475-101488 (2017). PubMed: 29254179
    • Okawa S  et al. Proteome and Secretome Characterization of Glioblastoma-Derived Neural Stem Cells. Stem Cells 35:967-980 (2017). PubMed: 27870168
    • Truchan HK  et al. Type II Secretion Substrates of Legionella pneumophila Translocate Out of the Pathogen-Occupied Vacuole via a Semipermeable Membrane. MBio 8:N/A (2017). ICC/IF . PubMed: 28634242
    • Ma Z  et al. Galectin-3 Inhibition Is Associated with Neuropathic Pain Attenuation after Peripheral Nerve Injury. PLoS One 11:e0148792 (2016). WB . PubMed: 26872020
    • DE Oliveira JT  et al. Differential expression of galectin-1 and galectin-3 in canine non-malignant and malignant mammary tissues and in progression to metastases in mammary tumors. Anticancer Res 34:2211-21 (2014). PubMed: 24778023
    • Petropolis DB  et al. A new human 3D-liver model unravels the role of galectins in liver infection by the parasite Entamoeba histolytica. PLoS Pathog 10:e1004381 (2014). ICC/IF ; Human . PubMed: 25211477
    • Cui GQ  et al. Proteomic analysis of meningiomas. Acta Neurol Belg 114:187-94 (2014). WB ; Human . PubMed: 24085542

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Galectin 3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (microglia)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    20 µg
    Specification
    microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 12 2018

    Question

    Dear sirs,

    I need the safety data sheet pdf Rabbit polyclonal to Galectin 3 ref. ab31707, please.

    Thank you very much,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2012

    Answer

    Thank you for contacting us.

    Please find the SDS document for the anti-Galectin 3 antibody (ab31707) attached to this email. This can now also be accessed from the datasheet of ab31707.

    I hope this information has been of help. If you have any further questions or requests, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Aug 21 2012

    Question

    Phone call requesting information of if the cross-reactivity of anti-Galectin 3 antibody (ab31707) is likely to detect the frog protein.

    Read More

    Abcam community

    Verified customer

    Asked on May 25 2012

    Answer

    Thank you for contacting us yesterday in regards to the anti-Galectin 3 antibody (ab31707).

    As discussed over the phone I have checked the sequence homology of the human protein (from which the immunogen of this antibody was taken) to that of the frog protein. Unfortunately the sequence homology is very low (49%) and I would therefore not expect the antibody to be able to detect the frog protein. As all of our Galectin 3 antibodies are raised against immunogens taken from the human ormouseprotein (which does not have any higherhomology to thefrog protein that thehuman)I am unfortunately unable to suggest a suitable alternative for you.

    I am sorry that I could not be of more help in this instance. If you have any further questions please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on May 25 2012

    Question

    I would like to test ab31707 for use in IP

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2011

    Answer

    DISCOUNT CODE: *** Expiration date: April 14th, 2012 I am very pleased to hear you would like to accept our offer and test ab31707 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2011

    Western blot abreview for Anti-Galectin 3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast carcinoma)
    Loading amount
    50 µg
    Specification
    Breast carcinoma
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Galectin 3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (HT1080)
    Specification
    HT1080
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 4%
    Read More

    Abcam user community

    Verified customer

    Submitted May 01 2008

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