The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
1/50 - 1/100.
Use at an assay dependent concentration. PubMed: 21652631
1/100 - 1/500.
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Contains 1 galectin domain.
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
Immunohistochemical analysis of paraffin-embedded
human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).
Western blot - Anti-Galectin 3 antibody (ab53082)
All lanes : Anti-Galectin 3 antibody (ab53082) at 1/500 dilution
Lane 1 : Hela cell extract with no blocking peptide Lane 2 : Hela cell extract with blocking peptide (ab53082 immunogen)
Predicted band size: 26 kDa Observed band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab53082)This image is courtesy of an anonymous Abreview.
Immunocytochemical analysis of mouse macrophage cells, labeling Galectin 3 with ab53082. Cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked in 1% BSA for 30 minutes at 37°C. Incubation with ab53082 (diluted 1/500 in PBS) was for 1 hour at 37°C. The secondary antibody used was ab150073 diluted 1/500.