Overview

  • Product name
  • Description
    Rabbit polyclonal to Galectin 3
  • Host species
    Rabbit
  • Specificity
    Galectin 3 antibody detects endogenous levels of total Galectin 3 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Galectin 3 (internal sequence). The immunogen is in the range of aa 141-190.
    Database link: P17931

  • Positive control
    • human lung carcinoma tissue; Hela cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 21652631
ICC/IF 1/100 - 1/500.

Target

  • Function
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificity
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similarities
    Contains 1 galectin domain.
  • Cellular localization
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa lectin antibody
    • Carbohydrate binding protein 35 antibody
    • Carbohydrate-binding protein 35 antibody
    • CBP 35 antibody
    • CBP35 antibody
    • Gal-3 antibody
    • GAL3 antibody
    • Galactose-specific lectin 3 antibody
    • Galactoside-binding protein antibody
    • GALBP antibody
    • Galectin 3 internal gene,included antibody
    • Galectin-3 antibody
    • Galectin3 antibody
    • GALIG antibody
    • GBP antibody
    • IgE binding protein antibody
    • IgE-binding protein antibody
    • L 31 antibody
    • L 34 antibody
    • L-31 antibody
    • L-34 galactoside-binding lectin antibody
    • L31 antibody
    • Laminin-binding protein antibody
    • Lectin L-29 antibody
    • Lectin, galactose binding, soluble 3 antibody
    • LEG3_HUMAN antibody
    • LGALS2 antibody
    • LGALS3 antibody
    • MAC 2 antigen antibody
    • Mac-2 antibody
    • Mac-2 antigen antibody
    • MAC2 antibody
    • Macrophage galactose-specific lectin antibody
    • MGC105387 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).
  • All lanes : Anti-Galectin 3 antibody (ab53082) at 1/500 dilution

    Lane 1 : Hela cell extract with no blocking peptide
    Lane 2 : Hela cell extract with blocking peptide (ab53082 immunogen)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunocytochemical analysis of mouse macrophage cells, labeling Galectin 3 with ab53082. Cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked in 1% BSA for 30 minutes at 37°C. Incubation with ab53082 (diluted 1/500 in PBS) was for 1 hour at 37°C. The secondary antibody used was ab150073 diluted 1/500.

    See Abreview

References

This product has been referenced in:
  • Jiang Q  et al. Erythrocyte-cancer hybrid membrane-camouflaged melanin nanoparticles for enhancing photothermal therapy efficacy in tumors. Biomaterials 192:292-308 (2019). Read more (PubMed: 30465973) »
  • Stojanovic B  et al. Galectin-3 Deficiency Facilitates TNF-a-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection. Front Microbiol 10:185 (2019). Read more (PubMed: 30800112) »
See all 19 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (1ry macrophages)
Permeabilization
Yes - Triton
Specification
1ry macrophages
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 17 2019

Abreviews
Application
Blocking
Sample
Human Cell (colorectal carcinoma)
Specification
colorectal carcinoma

Abcam user community

Verified customer

Submitted Oct 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Mouse Cell (Macrophage)
Specification
Macrophage
Permeabilization
Yes - Tx100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 08 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
No

Abcam user community

Verified customer

Submitted Aug 26 2010

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