Key features and details
- Rabbit monoclonal to Galectin 3 - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
Product nameAnti-Galectin 3 antibody - BSA and Azide free (Detector)
See all Galectin 3 primary antibodies
DescriptionRabbit monoclonal to Galectin 3 - BSA and Azide free (Detector)
Tested applicationsSuitable for: Sandwich ELISAmore details
ab270341 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey Galectin-3. The recommended pair for sandwich ELISA is:
Capture: ab270340, Human/Monkey Galectin-3 Capture Antibody (unconjugated)
Detector: ab270341, Human/Monkey Galectin-3 Detector Antibody (unconjugated)
The reference range value is 58.8 - 2000 ng/ml for human and 208.89 - 6000 pg/ml for monkey.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab270341 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).|
FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
- Information by UniProt
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
ab270341 has not yet been referenced specifically in any publications.