Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)
Key features and details
- Biotin Rat monoclonal [M3/38] to Galectin 3
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Galectin 3 antibody [M3/38]
See all Galectin 3 primary antibodies -
Description
Biotin Rat monoclonal [M3/38] to Galectin 3 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Galectin 3 of mouse origin
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Positive control
- Normal human peripheral blood cells, C57BL/6 Mouse bone marrow cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
M3/38 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.25µg for 105 cells.
ab95679 can be used at 0.25 µg/per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.25µg for 105 cells. ab95679 can be used at 0.25 µg/per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3958 Human
- Entrez Gene: 16854 Mouse
- Omim: 153619 Human
- SwissProt: P17931 Human
- SwissProt: P16110 Mouse
- Unigene: 531081 Human
- Unigene: 248615 Mouse
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Alternative names
- 35 kDa lectin antibody
- Carbohydrate binding protein 35 antibody
- Carbohydrate-binding protein 35 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab95679 has not yet been referenced specifically in any publications.