Product nameGalectin 3 overexpression 293T lysate (whole cell)
ab94225 is a 293T cell transfected lysate in which Human Galectin 3 has been transiently over-expressed using a pCMV-Galectin 3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. Tissue specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Similarity: Contains 1 galectin domain.
Our Abpromise guarantee covers the use of ab94225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94225 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-Galectin 3 antibody (ab58086) at 1/500 dilution
Lane 1 :
Galectin 3 overexpression 293T lysate (whole cell) (ab94225)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94225 has not yet been referenced specifically in any publications.