Key features and details
- Rabbit polyclonal to Galectin 7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Galectin 7 antibody
See all Galectin 7 primary antibodies
DescriptionRabbit polyclonal to Galectin 7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- WB: Recombinant Galectin 7 protein; HEK-293T transfected with Galectin 7, whole cell lysate. IP: HEK-293T transfected with Galectin 7, whole cell extract.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 15 kDa.|
|IP||1/100 - 1/500.|
FunctionCould be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
Tissue specificityMainly expressed in stratified squamous epithelium.
Sequence similaritiesContains 1 galectin domain.
Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
- Information by UniProt
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
Anti-Galectin 7 antibody (ab227553) at 1/90000 dilution + Recombinant Galectin 7 protein at 0.1 µg
Predicted band size: 15 kDa
All lanes : Anti-Galectin 7 antibody (ab227553) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Galectin 7, whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
15% SDS-PAGE gel.
Galectin 7 was immunoprecipitated from Galectin 7-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 μg ab227553. Western blot was performed from the immunoprecipitate using ab227553 at 1/500 dilution.
Lane 1: 4 µg preimmune Rabbit IgG instead of ab227553 in Galectin 7-transfected HEK-293T whole cell lysate.
Lane 2: ab227553 IP in Galectin 7-transfected HEK-293T whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227553 has not yet been referenced specifically in any publications.