Recombinant Anti-Galectin 7 antibody [EPR19903] (ab206435)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19903] to Galectin 7
- Suitable for: IHC-Fr, WB, IHC-P, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Galectin 7 antibody [EPR19903]
See all Galectin 7 primary antibodies -
Description
Rabbit monoclonal [EPR19903] to Galectin 7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, IP, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein within Human Galectin 7 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P47929 -
Positive control
- WB: Human, mouse and rat skin lysates; HaCaT whole cell lysate. IHC-P: Human skin and squamous carcinoma of the cervix tissues; mouse and rat skin tissues. IHC-Fr: Rat skin tissue. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells. IP: Human skin lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19903 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Buffer
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab206435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | 1/500. | |
WB | 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). | |
IHC-P | 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (ab93684) |
|
IP | 1/40. | |
ICC/IF | 1/500. | |
Flow Cyt | 1/600. |
Target
-
Function
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. -
Tissue specificity
Mainly expressed in stratified squamous epithelium. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
-
Database links
- Entrez Gene: 3963 Human
- Entrez Gene: 653499 Human
- Entrez Gene: 16858 Mouse
- Entrez Gene: 29518 Rat
- Omim: 600615 Human
- SwissProt: P47929 Human
- SwissProt: O54974 Mouse
- SwissProt: P97590 Rat
see all -
Alternative names
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
see all
Images
-
All lanes : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs such as skeletal muscle. PMID: 9697310; PMID: 7729568.
-
Lanes 1-2 : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution
Lane 3 : Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/1000 dilution
Lane 1 : Rat skin lysate
Lane 2 : Mouse skin lysate
Lane 3 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 30 seconds; Lane 3: 15 seconds.
Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs. PMID: 9697310; PMID: 7729568.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of human skin is observed (PMID: 23658821). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the cervix tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on tumor cells of squamous carcinoma of the cervix is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining in human kidney (PMID: 9697310).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of mouse skin is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining on mouse stomach (PMID: 9697310).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (ab206435)
Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of rat skin is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skin tissue labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on epithelium of rat skin is observed. Nuclear counterstain: DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HACAT cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
Flow cytometric analysis of 4% paraformaldehyde-fixed HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
-
Galectin 7 was immunoprecipitated from 0.35 mg of human skin lysate with ab206435 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab206435 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human skin lysate 10µg (Input).
Lane 2: ab206435 IP in human skin lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206435 in human skin lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab206435 has not yet been referenced specifically in any publications.