RecombinantRabMAb

Recombinant Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Flow Cytometry - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
  • Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19903] to Galectin 7 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free
    See all Galectin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR19903] to Galectin 7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein within Human Galectin 7 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P47929

  • General notes

    Ab240374 is the carrier-free version of ab206435. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240374 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(ab93684)
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

  • Tissue specificity

    Mainly expressed in stratified squamous epithelium.

  • Sequence similarities

    Contains 1 galectin domain.

  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Gal-7 antibody
    • GAL7 antibody
    • Galectin 7B antibody
    • Galectin-7 antibody
    • HKL-14 antibody
    • Human keratinocyte lectin 14 antibody
    • Keratinocyte lectin 14 antibody
    • Lectin galactoside binding soluble 7 antibody
    • Lectin, galactoside binding, soluble, 7B antibody
    • LEG7_HUMAN antibody
    • LGALS7 antibody
    • LGALS7A antibody
    • LGALS7B antibody
    • P53 induced protein 1 antibody
    • p53-induced gene 1 protein antibody
    • Pi7 antibody
    • PIG1 antibody
    • TP53I1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of human skin is observed (PMID: 23658821). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the cervix tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on tumor cells of squamous carcinoma of the cervix is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining in human kidney (PMID: 9697310).

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of mouse skin is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Negative control: No staining on mouse stomach (PMID: 9697310).

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of rat skin is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skin tissue labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on epithelium of rat skin is observed. Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HACAT cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

  • Flow Cytometry - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Flow Cytometry - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

  • Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

    Galectin 7 was immunoprecipitated from 0.35 mg of human skin lysate with ab206435 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab206435 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human skin lysate 10µg (Input).

    Lane 2: ab206435 IP in human skin lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206435 in human skin lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206435).

  • Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)
    Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free (ab240374)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240374? Please let us know so that we can cite the reference in this datasheet.

ab240374 has not yet been referenced specifically in any publications.

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