Recombinant
RabMAb

Recombinant Anti-Galectin 7 antibody [EPR4287] (Alexa Fluor® 647) (ab207047)

Overview

  • Product name

    Anti-Galectin 7 antibody [EPR4287] (Alexa Fluor® 647)
    See all Galectin 7 primary antibodies
  • Description

    Rabbit monoclonal [EPR4287] to Galectin 7 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Galectin 7 aa 1-100. The exact sequence is proprietary.
    Database link: P47929

  • Positive control

    • ICC/IF: A431 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.

This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
  • Tissue specificity

    Mainly expressed in stratified squamous epithelium.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gal-7 antibody
    • GAL7 antibody
    • Galectin 7B antibody
    • Galectin-7 antibody
    • HKL-14 antibody
    • Human keratinocyte lectin 14 antibody
    • Keratinocyte lectin 14 antibody
    • Lectin galactoside binding soluble 7 antibody
    • Lectin, galactoside binding, soluble, 7B antibody
    • LEG7_HUMAN antibody
    • LGALS7 antibody
    • LGALS7A antibody
    • LGALS7B antibody
    • P53 induced protein 1 antibody
    • p53-induced gene 1 protein antibody
    • Pi7 antibody
    • PIG1 antibody
    • TP53I1 antibody
    see all

Images

  • ab207047 staining Galectin 7 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207047 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in A431 cells fixed with 100% methanol (5 min)

References

ab207047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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