RecombinantRabMAb

Recombinant Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

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Western blot - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunocytochemistry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Flow Cytometry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
  • Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4287] to Galectin 7 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free
    See all Galectin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR4287] to Galectin 7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Galectin 7. The exact sequence is proprietary.

  • Positive control

    • ICC: HaCaT cells IP: HaCaT whole cell lysate.

  • General notes

    ab247704 is the carrier-free version of ab108623 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247704 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • Function

    Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

  • Tissue specificity

    Mainly expressed in stratified squamous epithelium.

  • Sequence similarities

    Contains 1 galectin domain.

  • Cellular localization

    Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
  • Information by UniProt

  • Database links

    • Alternative names

      • Gal-7 antibody
      • GAL7 antibody
      • Galectin 7B antibody
      • Galectin-7 antibody
      • HKL-14 antibody
      • Human keratinocyte lectin 14 antibody
      • Keratinocyte lectin 14 antibody
      • Lectin galactoside binding soluble 7 antibody
      • Lectin, galactoside binding, soluble, 7B antibody
      • LEG7_HUMAN antibody
      • LGALS7 antibody
      • LGALS7A antibody
      • LGALS7B antibody
      • P53 induced protein 1 antibody
      • p53-induced gene 1 protein antibody
      • Pi7 antibody
      • PIG1 antibody
      • TP53I1 antibody
      see all

    Images

    • Western blot - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Western blot - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      All lanes : Anti-Galectin 7 antibody [EPR4287] (ab108623) at 1/1000 dilution

      Lane 1 : Human skin lysate
      Lane 2 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa



      This data was developed using ab108623, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

      This data was developed using ab108623, the same antibody clone in a different buffer formulation.

      ab108623, at 1/500 dilution, staining Galectin 7 in paraffin-embedded Human skin tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunocytochemistry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using the same antibody clone in a different buffer formulation (ab108623).
      Immunocytochemistry analysis of HaCaT (human skin keratinocyte) labeling Galectin 7 with purified ab108623 at 1/1000 dilution (0.5 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.22 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Flow Cytometry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Flow Cytometry - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

      This data was developed using ab108623, the same antibody clone in a different buffer formulation.

      Flow cytometry analysis of HACAT (Human skin keratinocyte) cells labeling Galectin 7 (red) with ab108623 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

      This data was developed using ab108623, the same antibody clone in a different buffer formulation.
      Purified ab108623 at 1/50 dilution (2µg) immunoprecipitating Galectin 7 in HaCaT whole cell lysate.
      Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
      Lane 2 (+): ab108623 + HaCaT whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108623 in HaCaT whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 15 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using ab108623, the same antibody clone in a different buffer formulation.ab108623 showing negative staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using ab108623, the same antibody clone in a different buffer formulation.ab108623 showing negative staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using ab108623, the same antibody clone in a different buffer formulation.ab108623 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using ab108623, the same antibody clone in a different buffer formulation.ab108623 showing negative staining in Normal heart tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      This data was developed using ab108623, the same antibody clone in a different buffer formulation.ab108623 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)
      Anti-Galectin 7 antibody [EPR4287] - BSA and Azide free (ab247704)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247704? Please let us know so that we can cite the reference in this datasheet.

    ab247704 has not yet been referenced specifically in any publications.

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