Product nameAnti-Galectin 8 / Gal-8 antibody
See all Galectin 8 / Gal-8 primary antibodies
DescriptionRabbit polyclonal to Galectin 8 / Gal-8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey
Synthetic peptide corresponding to Galectin 8/ Gal-8 aa 150-250 conjugated to keyhole limpet haemocyanin.
- This antibody gave a positive signal in Galectin 8 / Gal-8 Tagged Recombinant Protein.
Previously labelled as Galectin 8.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Tissue specificityUbiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
- Information by UniProt
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
Anti-Galectin 8 / Gal-8 antibody (ab42879) at 1 µg/ml + Galectin 8 / Gal-8 Recombinant Protein at 0.1 µg
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab42879 recognizes the full length tagged recombinant Galectin 8 / Gal-8 protein which has an expected molecular weight of 65.5 kDa.
ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab42879 has not yet been referenced specifically in any publications.