Overview

  • Product name
    Anti-Galectin 8 / Gal-8 antibody
    See all Galectin 8 / Gal-8 primary antibodies
  • Description
    Rabbit polyclonal to Galectin 8 / Gal-8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Galectin 8/ Gal-8 aa 150-250 conjugated to keyhole limpet haemocyanin.

  • Positive control
    • This antibody gave a positive signal in Galectin 8 / Gal-8 Tagged Recombinant Protein.
  • General notes

    Previously labelled as Galectin 8. 

Properties

Applications

Our Abpromise guarantee covers the use of ab42879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Anti-Galectin 8 / Gal-8 antibody (ab42879) at 1 µg/ml + Galectin 8 / Gal-8 Recombinant Protein at 0.1 µg

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    ab42879 recognizes the full length tagged recombinant Galectin 8 / Gal-8 protein which has an expected molecular weight of 65.5 kDa.

  • ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References

ab42879 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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