Product nameAnti-Galectin 8/Gal-8 antibody
See all Galectin 8/Gal-8 primary antibodies
DescriptionRabbit polyclonal to Galectin 8/Gal-8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey
Synthetic peptide corresponding to Galectin 8/Gal-8 aa 150-250 conjugated to keyhole limpet haemocyanin.
- This antibody gave a positive signal in Galectin 8/Gal-8 Tagged Recombinant Protein.
Previously labelled as Galectin 8.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Tissue specificityUbiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
- Information by UniProt
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
Anti-Galectin 8/Gal-8 antibody (ab42879) at 1 µg/ml + Galectin 8/Gal-8 Recombinant Protein at 0.1 µg
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab42879 recognizes the full length tagged recombinant Galectin 8/Gal-8 protein which has an expected molecular weight of 65.5 kDa.
ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab42879 has not yet been referenced specifically in any publications.