Key features and details
- Rabbit polyclonal to Galectin 8/Gal-8
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Galectin 8/Gal-8 antibody
See all Galectin 8/Gal-8 primary antibodies
DescriptionRabbit polyclonal to Galectin 8/Gal-8
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Galectin 8/Gal-8 (internal sequence).
Previously labelled as Galectin 8.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects bands of approximately 36 and 60 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityUbiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
- Information by UniProt
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
All lanes : Anti-Galectin 8/Gal-8 antibody (ab69631) at 1/500 dilution
Lane 1 : extracts of 3T3 cells
Lane 2 : extracts of 3T3 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1:50 dilution.Left image un-treated. Right image treated with immunizing peptide.
ab69631 has been referenced in 2 publications.
- Reticker-Flynn NE & Bhatia SN Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche. Cancer Discov 5:168-81 (2015). PubMed: 25421439
- Reticker-Flynn NE et al. A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis. Nat Commun 3:1122 (2012). WB, IHC ; Mouse . PubMed: 23047680