Overview

  • Product name

    Anti-Galectin 8/Gal-8 antibody
    See all Galectin 8/Gal-8 primary antibodies
  • Description

    Rabbit polyclonal to Galectin 8/Gal-8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Galectin 8/Gal-8 (internal sequence).

  • Positive control

    • Extracts from 3T3 cells and human prostate carcinoma tissue.
  • General notes

    Previously labelled as Galectin 8. 

Properties

Applications

Our Abpromise guarantee covers the use of ab69631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects bands of approximately 36 and 60 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity

      Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
    • Sequence similarities

      Contains 2 galectin domains.
    • Domain

      Contains two homologous but distinct carbohydrate-binding domains.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Gal 8 antibody
      • Gal-8 antibody
      • Gal8 antibody
      • Galectin 8 antibody
      • Galectin-8 antibody
      • galectin-8g antibody
      • Lectin galactoside binding soluble 8 antibody
      • LEG8_HUMAN antibody
      • LGAL S8 antibody
      • Lgals8 antibody
      • PCTA 1 antibody
      • PCTA-1 antibody
      • PCTA1 antibody
      • Po66 carbohydrate binding protein antibody
      • Po66 carbohydrate-binding protein antibody
      • Po66 CBP antibody
      • Po66-CBP antibody
      • Prostate carcinoma tumor antigen 1 antibody
      see all

    Images

    • All lanes : Anti-Galectin 8/Gal-8 antibody (ab69631) at 1/500 dilution

      Lane 1 : extracts of 3T3 cells
      Lane 2 : extracts of 3T3 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 36 kDa
      Observed band size: 36 kDa
      Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1:50 dilution.Left image un-treated. Right image treated with immunizing peptide.

    References

    This product has been referenced in:

    • Reticker-Flynn NE & Bhatia SN Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche. Cancer Discov 5:168-81 (2015). Read more (PubMed: 25421439) »
    • Reticker-Flynn NE  et al. A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis. Nat Commun 3:1122 (2012). WB, IHC ; Mouse . Read more (PubMed: 23047680) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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