Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4857] to Galectin 8/Gal-8
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-Galectin 8/Gal-8 antibody [EPR4857]
See all Galectin 8/Gal-8 primary antibodies
DescriptionRabbit monoclonal [EPR4857] to Galectin 8/Gal-8
Specificityab109519 has weak cross-reactivity with Galectin 9.
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Galectin 8/Gal-8 aa 1-100. The exact sequence is proprietary.
- HepG2 or LNCaP cell lysate Human prostatic adenocarcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Galectin 8.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
Tissue specificityUbiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
- Information by UniProt
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/10000 dilution (purified) + LNCAP (human prostat carcinoma) whole cell lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Blocking and diluting buffer 5% NFDM/TBST
ab109519 staining Galectin 8/Gal-8 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab109519 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
ab109519 staining Galectin 8/Gal-8 in the human cell line HEK293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
All lanes : Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Lanes 1 & 3 : unpurified at 1/1000 dilution
Lane 2 : Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/200 dilution (unpurified)
All lanes : Galectin 9 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 40 kDa
ab109519 has weak cross-reactivity with Galectin 9.
ab109519 staining Galectin 8/Gal-8 in human prostatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
Unpurified ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Overlay histogram showing HeLa cells stained with unpurified ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109519, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab109519 has been referenced in 7 publications.
- Trebo A et al. High Galectin-7 and Low Galectin-8 Expression and the Combination of both are Negative Prognosticators for Breast Cancer Patients. Cancers (Basel) 12:N/A (2020). PubMed: 32290551
- Fraser J et al. Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. EMBO Rep 20:e47734 (2019). PubMed: 31448519
- Schulz H et al. Overall Survival of Ovarian Cancer Patients Is Determined by Expression of Galectins-8 and -9. Int J Mol Sci 19:N/A (2018). IHC-P ; Human . PubMed: 29361803
- Teo WX et al. MTMR4 Is Required for the Stability of the Salmonella-Containing Vacuole. Front Cell Infect Microbiol 6:91 (2016). PubMed: 27625994
- Copits BA et al. Modulation of ionotropic glutamate receptor function by vertebrate galectins. J Physiol 592:2079-96 (2014). Rat . PubMed: 24614744
- Lee YJ et al. Spatial and temporal expression, and statin responsiveness of galectin-1 and galectin-3 in murine atherosclerosis. Korean Circ J 43:223-30 (2013). IHC-FoFr ; Mouse . PubMed: 23682281
- Miwa HE et al. Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer. Glycobiology 23:1477-90 (2013). Mouse . PubMed: 24037315