Recombinant Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4857] to Galectin 8/Gal-8 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free
See all Galectin 8/Gal-8 primary antibodies -
Description
Rabbit monoclonal [EPR4857] to Galectin 8/Gal-8 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody has weak cross-reactivity with Galectin 9.
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Tested applications
Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa whole cell lysate.
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General notes
ab239983 is the carrier-free version of ab109519.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4857 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)
- PE Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab305934)
- APC Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab305935)
- HRP Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab305936)
- Alexa Fluor® 488 Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab309695)
- Alexa Fluor® 647 Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab310062)
- Alexa Fluor® 594 Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab310457)
- Alexa Fluor® 555 Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab311986)
- Alexa Fluor® 568 Anti-Galectin 8 / Gal-8 antibody [EPR4857] (ab312461)
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Tissue specificity
Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3964 Human
- Omim: 606099 Human
- SwissProt: O00214 Human
- Unigene: 4082 Human
- Unigene: 708114 Human
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Alternative names
- Gal 8 antibody
- Gal-8 antibody
- Gal8 antibody
see all
Images
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Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/10000 dilution (purified) + LNCAP (human prostat carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
Blocking and diluting buffer 5% NFDM/TBST
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All lanes : Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : LNCaP cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
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Lanes 1 & 3 : unpurified at 1/1000 dilution
Lane 2 : Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/200 dilution (unpurified)
All lanes : Galectin 9 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 40 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
ab109519 has weak cross-reactivity with Galectin 9.
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ab109519 staining Galectin 8/Gal-8 in the human cell line HEK293 (human embryonic kidney) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
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ab109519 staining Galectin 8/Gal-8 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab109519 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
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ab109519 staining Galectin 8/Gal-8 in human prostatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
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This data was developed using ab109519, the same antibody clone in a different buffer formulation.
Purified ab109519 at 1/30 dilution (2µg) immunoprecipitating Galectin 8/Gal-8 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109519 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109519 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36/40 kDa -
Unpurified ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Overlay histogram showing HeLa cells stained with unpurified ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109519, 1/100) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109519).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239983 has not yet been referenced specifically in any publications.