• Product name
  • Description
    Rabbit polyclonal to galectin 9
  • Host species
  • Specificity
    This antibody detects endogenous levels of total Galectin-9 protein.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human galectin 9 aa 51-100 (internal sequence).
    Database link: O00182

  • Positive control
    • NIH/3T3 cells. Extracts from HepG2 cells, human lymphoma



Our Abpromise guarantee covers the use of ab69630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.


  • Function
    Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificity
    Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similarities
    Contains 2 galectin domains.
  • Domain
    Contains two homologous but distinct carbohydrate-binding domains.
  • Cellular localization
    Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa beta-galactoside-binding lectin antibody
    • Ecalectin antibody
    • Gal-9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • UAT antibody
    • Urate transporter/channel antibody
    • Urate transporter/channel protein antibody
    see all


  • Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
  • All lanes : Anti-galectin 9 antibody (ab69630) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Ab69630 recognizes the partial tagged recombinant Human galectin 9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
  • ab69360 (4µg/ml) staining Galectin 9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Labrie M  et al. Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers. Sci Rep 7:13244 (2017). Read more (PubMed: 29038585) »
  • Hou N  et al. T-cell immunoglobulin and mucin domain-containing protein-3 and galectin-9 protein expression: Potential prognostic significance in esophageal squamous cell carcinoma for Chinese patients. Oncol Lett 14:8007-8013 (2017). Read more (PubMed: 29344243) »

See all 5 Publications for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Mouse Cell (Neurons)
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Gokhan Yilmaz

Verified customer

Submitted Sep 21 2016

Thank you for contacting us.

I am confirming that I have logged your phone call in our system. If you have any future inquiry please do not hesitate to call us back again.

I hope this information is helpful to you. Please do not hesit...

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Thank you for your reply.

Please let me know if you have any success by changing the blocking buffer. It is actually not that uncommon to load such amounts of whole cell lysates, however if that is not feasible for your experiments I am...

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Thank you for contacting Abcam regarding ab69630.

I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to impro...

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Thank you for your reply.

We did not test this antibody with a standard protein.This antibody can be used for ELISA due to validation by the following:

1) Peptide-Based ELISA: Coat immunogen peptide in 96-well plate. Det...

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Thank you once again for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As requested, I have obtained a copy of the testing protocol. Please bear in mind that this...

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Thank you for contacting Abcam regarding ab69630.

Regarding our testing conditions for ELISA, this was tested for immunogen-coated ELISA using a conjugated secondary antibody. It has not been tested in sandwich ELISA.


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Thank you for your enquiry. I have contacted the originator of the product, but unfortunately, there is only limited amount of the peptide and is not available for purchase. I am sorry I can't be of more assistance in this occasion but if there...

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