Anti-galectin 9/Gal-9 antibody [1G3] (ab153673)
Key features and details
- Mouse monoclonal [1G3] to galectin 9/Gal-9
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-galectin 9/Gal-9 antibody [1G3]
See all galectin 9/Gal-9 primary antibodies -
Description
Mouse monoclonal [1G3] to galectin 9/Gal-9 -
Host species
Mouse -
Specificity
This antibody fails to detect endogenous natural samples in WB.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human galectin 9/Gal-9 (C terminal). Recombinant fragment produced in E. coli
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Positive control
- WB: HeLa transfected with galectin 9 / Gal-9 whole cell lysate IHC-P: Human normal Tonsil tissue
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1G3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 3965 Human
- Omim: 601879 Human
- SwissProt: O00182 Human
- Unigene: 81337 Human
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Alternative names
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
Images
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IHC image of galectin 9/Gal-9 staining in Human normal Tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153673, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-galectin 9/Gal-9 antibody [1G3] (ab153673) at 5 µg/ml
Lane 1 : HeLa transfected with galectin 9 / Gal-9 whole cell lysate
Lane 2 : HeLa (wild type control) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Additional bands at: 18 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab153673 has been referenced in 8 publications.
- Yoshikawa K et al. Prognostic significance of the expression levels of T-cell immunoglobulin mucin-3 and its ligand galectin-9 for relapse-free survival in triple-negative breast cancer. Oncol Lett 23:197 (2022). PubMed: 35572493
- Wang X et al. An Integrated Systematic Analysis and the Clinical Significance of Hepcidin in Common Malignancies of the Male Genitourinary System. Front Genet 13:771344 (2022). PubMed: 35646093
- Curley J et al. Looking past PD-L1: expression of immune checkpoint TIM-3 and its ligand galectin-9 in cervical and vulvar squamous neoplasia. Mod Pathol 33:1182-1192 (2020). PubMed: 32139873
- Xu D et al. PD-L1 Expression Is Regulated By NF-?B During EMT Signaling In Gastric Carcinoma. Onco Targets Ther 12:10099-10105 (2019). PubMed: 31819504
- Schulz H et al. Overall Survival of Ovarian Cancer Patients Is Determined by Expression of Galectins-8 and -9. Int J Mol Sci 19:N/A (2018). IHC-P ; Human . PubMed: 29361803
- Enninga EAL et al. CD206-positive myeloid cells bind galectin-9 and promote a tumor-supportive microenvironment. J Pathol 245:468-477 (2018). PubMed: 29732570
- Feng J et al. Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20. J Virol 92:N/A (2018). PubMed: 29695422
- Barjon C et al. A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses. Infect Agent Cancer 7:16 (2012). WB, IHC ; Mouse . PubMed: 22805533