Overview

  • Product name

    Anti-galectin 9/Gal-9 antibody [1G3]
    See all galectin 9/Gal-9 primary antibodies
  • Description

    Mouse monoclonal [1G3] to galectin 9/Gal-9
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human galectin 9/Gal-9 (C terminal). Recombinant fragment produced in E. coli

  • Positive control

    • Recombinant galectin 9/Gal-9 protein This antibody gave a positive result in IHC in the following FFPE tissue: Human normal Tonsil
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Previously labelled as galectin 9. 

Properties

Applications

Our Abpromise guarantee covers the use of ab153673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificity

    Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similarities

    Contains 2 galectin domains.
  • Domain

    Contains two homologous but distinct carbohydrate-binding domains.
  • Cellular localization

    Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36 kDa beta-galactoside-binding lectin antibody
    • Ecalectin antibody
    • Gal-9 antibody
    • galectin 9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • UAT antibody
    • Urate transporter/channel antibody
    • Urate transporter/channel protein antibody
    see all

Images

  • IHC image of galectin 9/Gal-9 staining in Human normal Tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153673, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-galectin 9/Gal-9 antibody [1G3] (ab153673) at 5 µg/ml

    Lane 1 : HeLa transfected with galectin 9 / Gal-9 whole cell lysate
    Lane 2 : HeLa (wild type control) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Additional bands at: 18 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References

This product has been referenced in:

  • Schulz H  et al. Overall Survival of Ovarian Cancer Patients Is Determined by Expression of Galectins-8 and -9. Int J Mol Sci 19:N/A (2018). IHC-P ; Human . Read more (PubMed: 29361803) »
  • Enninga EAL  et al. CD206-positive myeloid cells bind galectin-9 and promote a tumor-supportive microenvironment. J Pathol 245:468-477 (2018). Read more (PubMed: 29732570) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human glioblastoma cell)
Total protein in input
40 µg
Immuno-precipitation step
Protein A/G
Specification
human glioblastoma cell

Taeho Ko

Verified customer

Submitted Aug 01 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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