Anti-galectin 9/Gal-9 antibody (ab69630)
Key features and details
- Rabbit polyclonal to galectin 9/Gal-9
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-galectin 9/Gal-9 antibody
See all galectin 9/Gal-9 primary antibodies -
Description
Rabbit polyclonal to galectin 9/Gal-9 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human galectin 9/Gal-9 aa 51-100 (internal sequence).
Database link: O00182 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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|
IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
|
ICC/IF | (1) |
1/500 - 1/1000.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 3965 Human
- Entrez Gene: 16859 Mouse
- Entrez Gene: 25476 Rat
- Omim: 601879 Human
- SwissProt: O00182 Human
- SwissProt: O08573 Mouse
- SwissProt: P97840 Rat
- Unigene: 81337 Human
see all -
Alternative names
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
Images
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Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
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All lanes : Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Ab69630 recognizes the partial tagged recombinant Human galectin 9/Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab69630 has been referenced in 28 publications.
- Grabowska K et al. HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages. J Neurovirol 28:99-112 (2022). PubMed: 35175539
- Beyer S et al. Galectin-8 and -9 as prognostic factors for cervical cancer. Arch Gynecol Obstet 306:1211-1220 (2022). PubMed: 35377045
- Wu C et al. Single-cell characterization of malignant phenotypes and microenvironment alteration in retinoblastoma. Cell Death Dis 13:438 (2022). PubMed: 35523772
- Guo Q et al. Cancer cell intrinsic TIM-3 induces glioblastoma progression. iScience 25:105329 (2022). PubMed: 36325060
- Wang W et al. Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation. J Immunol 206:2692-2699 (2021). PubMed: 33963043