Overview

  • Product name
    Anti-galectin 9/Gal-9 antibody
    See all galectin 9/Gal-9 primary antibodies
  • Description
    Rabbit polyclonal to galectin 9/Gal-9
  • Host species
    Rabbit
  • Specificity

    This antibody detects endogenous levels of total galectin 9/Gal-9 protein.

  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human galectin 9/Gal-9 aa 51-100 (internal sequence).
    Database link: O00182

  • Positive control
    • NIH/3T3 cells. Extracts from HepG2 cells, human lymphoma
  • General notes

    Previously labelled as galectin 9. 

Properties

Applications

Our Abpromise guarantee covers the use of ab69630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificity
    Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similarities
    Contains 2 galectin domains.
  • Domain
    Contains two homologous but distinct carbohydrate-binding domains.
  • Cellular localization
    Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa beta-galactoside-binding lectin antibody
    • Ecalectin antibody
    • Gal-9 antibody
    • galectin 9 antibody
    • Galectin-9 antibody
    • galectin9 antibody
    • HOM HD 21 antibody
    • HOMHD21 antibody
    • HUAT antibody
    • Lectin galactoside binding soluble 9 antibody
    • LEG9_HUMAN antibody
    • LGAL S9 antibody
    • LGALS 9 antibody
    • Lgals9 antibody
    • LGALS9A antibody
    • MGC117375 antibody
    • MGC125973 antibody
    • MGC125974 antibody
    • Tumor antigen HOM-HD-21 antibody
    • UAT antibody
    • Urate transporter/channel antibody
    • Urate transporter/channel protein antibody
    see all

Images

  • Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
  • All lanes : Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Ab69630 recognizes the partial tagged recombinant Human galectin 9/Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.

  • ab69360 (4µg/ml) staining galectin 9 / Gal-9 in human lymphoma using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and secreted staining throughout the tumour.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Huang N  et al. Spleen-Associated Effects on Immunity in Hepatitis B Virus-Related Cirrhosis with Portal Hypertension. J Interferon Cytokine Res 39:95-105 (2019). Read more (PubMed: 30676849) »
  • Labrie M  et al. Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers. Sci Rep 7:13244 (2017). Read more (PubMed: 29038585) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neurons)
Specification
Neurons
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde

Gokhan Yilmaz

Verified customer

Submitted Sep 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up