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  1. Link

    galectin-9gal-9-antibody-epr23853-132-ab275877.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
  • Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
  • Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
  • Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-galectin 9/Gal-9 antibody [EPR23853-132]
    See all galectin 9/Gal-9 primary antibodies
  • Description

    Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus, liver and lung tissue lysates; EL4.IL-2, RAW 264.7 and NIH/3T3 whole cell lysates; Recombinant mouse galectin 9/Gal-9 protein. ICC: EL4.IL-2 cells. Flow Cyt (intra): EL4.IL-2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23853-132
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Neuroscience
    • Cell Adhesion Proteins
    • Nuclear Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • Anti-galectin 9/Gal-9 antibody [EPR23853-132] - BSA and Azide free (ab275886)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab275877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/50.
WB
1/1000. Detects a band of approximately 15, 36, 40 kDa (predicted molecular weight: 40 kDa).
Flow Cyt (Intra)
1/500.
Notes
ICC
1/50.
WB
1/1000. Detects a band of approximately 15, 36, 40 kDa (predicted molecular weight: 40 kDa).
Flow Cyt (Intra)
1/500.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
  • Tissue specificity

    Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
  • Sequence similarities

    Contains 2 galectin domains.
  • Domain

    Contains two homologous but distinct carbohydrate-binding domains.
  • Cellular localization

    Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.
  • Target information above from: UniProt accession O00182 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 16859 Mouse
    • SwissProt: O08573 Mouse
    • Unigene: 341434 Mouse
    • Alternative names

      • 36 kDa beta-galactoside-binding lectin antibody
      • Ecalectin antibody
      • Gal-9 antibody
      • galectin 9 antibody
      • Galectin-9 antibody
      • galectin9 antibody
      • HOM HD 21 antibody
      • HOMHD21 antibody
      • HUAT antibody
      • Lectin galactoside binding soluble 9 antibody
      • LEG9_HUMAN antibody
      • LGAL S9 antibody
      • LGALS 9 antibody
      • Lgals9 antibody
      • LGALS9A antibody
      • MGC117375 antibody
      • MGC125973 antibody
      • MGC125974 antibody
      • Tumor antigen HOM-HD-21 antibody
      • UAT antibody
      • Urate transporter/channel antibody
      • Urate transporter/channel protein antibody
      see all

    Images

    • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      All lanes : Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse lung tissue lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
      Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      Lanes 1-4 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
      Lanes 5-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 40 kDa
      Observed band size: 15,36,40 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Bands of 40KDa and 36KDa respectively represent two different isoforms.

      Band of 15KDa may represents the cleaved form.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233, 11886844, 22805533, 15811318).

      Low expression: mouse brain (PMID: 9038233).

      Samples are non-boiled as boiling may cause protein aggregates.

      Exposure time: Lanes 1-2: 5.5 seconds; Lanes 3-6: 37 seconds; Lanes 7: 3 minutes.

       

    • Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 cells labelling galectin 9/Gal-9 with ab275877 at 1/50 (12.28 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in EL4.IL-2 cell line. The staining pattern observed is consistent with what has been described in the literature (PMID: 28750861). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) cells labelling galectin 9/Gal-9 with ab275877 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution + Recombinant mouse galectin 9/Gal-9 protein, 20 ng

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 40 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds.

       

    • Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
      Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab275877? Please let us know so that we can cite the reference in this datasheet.

    ab275877 has not yet been referenced specifically in any publications.

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