Recombinant Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
![Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)](https://www.abcam.com/ps/products/275/ab275877/Images/ab275877-411823-anti-galectin-9gal9-antibody-epr23853132-western-blot-lysate.jpg "Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9
- Suitable for: ICC, WB, Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-galectin 9/Gal-9 antibody [EPR23853-132]
See all galectin 9/Gal-9 primary antibodies -
Description
Rabbit monoclonal [EPR23853-132] to galectin 9/Gal-9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus, liver and lung tissue lysates; EL4.IL-2, RAW 264.7 and NIH/3T3 whole cell lysates; Recombinant mouse galectin 9/Gal-9 protein. ICC: EL4.IL-2 cells. Flow Cyt (intra): EL4.IL-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23853-132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/50.
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| WB |
1/1000. Detects a band of approximately 15, 36, 40 kDa (predicted molecular weight: 40 kDa).
|
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| Flow Cyt (Intra) |
1/500.
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| Notes |
|---|
|
ICC
1/50. |
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WB
1/1000. Detects a band of approximately 15, 36, 40 kDa (predicted molecular weight: 40 kDa). |
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Flow Cyt (Intra)
1/500. |
Target
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 16859 Mouse
- SwissProt: O08573 Mouse
- Unigene: 341434 Mouse
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Alternative names
- 36 kDa beta-galactoside-binding lectin antibody
- Ecalectin antibody
- Gal-9 antibody
see all
Images
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Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)All lanes : Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : EL4.IL-2 (mouse lymphoma t lymphocyte) whole cell lysate
Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Lanes 5-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 15,36,40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Bands of 40KDa and 36KDa respectively represent two different isoforms.
Band of 15KDa may represents the cleaved form.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9038233, 11886844, 22805533, 15811318).
Low expression: mouse brain (PMID: 9038233).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: Lanes 1-2: 5.5 seconds; Lanes 3-6: 37 seconds; Lanes 7: 3 minutes.
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![Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)](https://www.abcam.com/ps/products/275/ab275877/Images/ab275877-1-anti-galectin-9gal9-antibody-epr23853132-immunocytochemistry-el4il2.jpg)
Immunocytochemistry - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL-2 cells labelling galectin 9/Gal-9 with ab275877 at 1/50 (12.28 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in EL4.IL-2 cell line. The staining pattern observed is consistent with what has been described in the literature (PMID: 28750861). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)](https://www.abcam.com/ps/products/275/ab275877/Images/ab275877-2-anti-galectin-9gal9-antibody-epr23853132-flow-cytometry-el4il2.jpg)
Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized EL4.IL-2 (mouse lymphoma T lymphocyte) cells labelling galectin 9/Gal-9 with ab275877 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)](https://www.abcam.com/ps/products/275/ab275877/Images/ab275877-411824-anti-galectin-9gal9-antibody-epr23853132-western-blot-9gal9.jpg)
Western blot - Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877) at 1/1000 dilution + Recombinant mouse galectin 9/Gal-9 protein, 20 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 40 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
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![Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)](https://www.abcam.com/ps/products/275/ab275877/Images/ab275877-6-benefits-of-recombinant-antibodies.png)
Anti-galectin 9/Gal-9 antibody [EPR23853-132] (ab275877)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab275877 has not yet been referenced specifically in any publications.
