Key features and details
- Rabbit polyclonal to GALK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GALK1 antibody
See all GALK1 primary antibodies
DescriptionRabbit polyclonal to GALK1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog
Recombinant fragment (internal sequence). The exact sequence is proprietary.
Database link: P51570
- WB: HepG2 whole cell lysate. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMajor enzyme for galactose metabolism.
PathwayCarbohydrate metabolism; galactose metabolism.
Involvement in diseaseDefects in GALK1 are the cause of galactosemia II (GALCT2) [MIM:230200]. Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.
Sequence similaritiesBelongs to the GHMP kinase family. GalK subfamily.
- Information by UniProt
- EC 188.8.131.52 antibody
- epididymis secretory protein Li 19 antibody
- Galactokinase 1 antibody
Anti-GALK1 antibody (ab228533) at 1/2000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GALK1 (green) using ab228533 at 1:500 dilution in ICC/IF. Cells were fixed in 100% methanol for 5 minutes.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228533 has not yet been referenced specifically in any publications.