Anti-GALK2 antibody (ab153815)
Key features and details
- Rabbit polyclonal to GALK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GALK2 antibody -
Description
Rabbit polyclonal to GALK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 67-331 of Human GALK2.
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Positive control
- HepG2 and mouse kidney whole cell lysates; Hep3B cells; MCF7 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Acts on GalNAc. Also acts as a galactokinase when galactose is present at high concentrations. May be involved in a salvage pathway for the reutilization of free GalNAc derived from the degradation of complex carbohydrates. -
Sequence similarities
Belongs to the GHMP kinase family. GalK subfamily. - Information by UniProt
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Database links
- Entrez Gene: 2585 Human
- Entrez Gene: 69976 Mouse
- Omim: 137028 Human
- SwissProt: Q01415 Human
- SwissProt: Q68FH4 Mouse
- Unigene: 122006 Human
- Unigene: 738080 Human
- Unigene: 738534 Human
see all -
Alternative names
- GALK2 antibody
- GaINAc Kinase antibody
- Galactokinase 2 antibody
see all
Images
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Anti-GALK2 antibody (ab153815) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10 % SDS-PAGE -
Anti-GALK2 antibody (ab153815) at 1/1000 dilution + Mouse kidney whole cell lysate at 50 µg
Predicted band size: 50 kDa
10 % SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labeling GALK2 with ab153815 at 1/500 dilution.
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Immunofluorescent analysis of methanol-fixed Hep3B cells labeling GALK2 with ab153815 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab153815 has been referenced in 1 publication.
- Xing J et al. Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota. Nat Commun 9:2020 (2018). PubMed: 29789585