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    galnt1-antibody-ab109918.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-GALNT1 antibody (ab109918)

  • Datasheet
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Western blot - Anti-GALNT1 antibody (ab109918)

    Key features and details

    • Rabbit polyclonal to GALNT1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GALNT1 antibody
      See all GALNT1 primary antibodies
    • Description

      Rabbit polyclonal to GALNT1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
    • Immunogen

      Synthetic peptide corresponding to Human GALNT1 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab156887)

    • Positive control

      • This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab109918 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/250. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa).

    Target

    • Function

      Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.
    • Tissue specificity

      Widely expressed. Expressed in all tissues tested.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
      Contains 1 ricin B-type lectin domain.
    • Domain

      There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
      The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.
    • Cellular localization

      Secreted and Golgi apparatus > Golgi stack membrane.
    • Target information above from: UniProt accession Q10472 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2589 Human
      • Entrez Gene: 14423 Mouse
      • Entrez Gene: 79214 Rat
      • Omim: 602273 Human
      • SwissProt: Q10472 Human
      • SwissProt: O08912 Mouse
      • SwissProt: Q10473 Rat
      • Unigene: 514806 Human
      • Unigene: 30249 Mouse
      • Unigene: 10266 Rat
      see all
    • Alternative names

      • GALNAC T1 antibody
      • GalNAc transferase 1 antibody
      • GalNAc-T1 antibody
      • GALNT 1 antibody
      • GALNT1 antibody
      • GALT1_HUMAN antibody
      • Polypeptide GalNAc transferase 1 antibody
      • Polypeptide N acetylgalactosaminyltransferase 1 antibody
      • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
      • pp GaNTase 1 antibody
      • pp-GaNTase 1 antibody
      • Protein UDP acetylgalactosaminyltransferase 1 antibody
      • Protein-UDP acetylgalactosaminyltransferase 1 antibody
      • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
      • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
      • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
      see all

    Images

    • Western blot - Anti-GALNT1 antibody (ab109918)
      Western blot - Anti-GALNT1 antibody (ab109918)
      All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution

      Lane 1 : Human kidney tissue lysate - total protein (ab30203)
      Lane 2 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 64 kDa


      Exposure time: 20 minutes


      We hypothesize that the band observed at 59 kDa represents the soluble form of GALNT1 due to the presence of a 40 amino acid signal peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109918 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab109918? Please let us know so that we can cite the reference in this datasheet.

    ab109918 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-GALNT1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    HepG2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2016

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