Anti-GALNT1 antibody (ab109918)

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Overview

  • Product name

    Anti-GALNT1 antibody
    See all GALNT1 primary antibodies

  • Description

    Rabbit polyclonal to GALNT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster

  • Immunogen

    Synthetic peptide corresponding to Human GALNT1 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156887)

  • Positive control

    • This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa).

Target

  • Function

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7.

  • Tissue specificity

    Widely expressed. Expressed in all tissues tested.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.

  • Domain

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.

  • Cellular localization

    Secreted and Golgi apparatus > Golgi stack membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • GALNAC T1 antibody
    • GalNAc transferase 1 antibody
    • GalNAc-T1 antibody
    • GALNT 1 antibody
    • GALNT1 antibody
    • GALT1_HUMAN antibody
    • Polypeptide GalNAc transferase 1 antibody
    • Polypeptide N acetylgalactosaminyltransferase 1 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 1 soluble form antibody
    • pp GaNTase 1 antibody
    • pp-GaNTase 1 antibody
    • Protein UDP acetylgalactosaminyltransferase 1 antibody
    • Protein-UDP acetylgalactosaminyltransferase 1 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 (GalNAc T1) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 1 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 antibody
    see all

Images

  • Western blot - Anti-GALNT1 antibody (ab109918)
    Western blot - Anti-GALNT1 antibody (ab109918)
    All lanes : Anti-GALNT1 antibody (ab109918) at 1/250 dilution

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa


    Exposure time: 20 minutes


    We hypothesize that the band observed at 59 kDa represents the soluble form of GALNT1 due to the presence of a 40 amino acid signal peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109918 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab109918 has not yet been referenced specifically in any publications.

Publishing research using ab109918? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-GALNT1 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Mar 30 2016

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