Key features and details
- Rabbit polyclonal to GALNT10
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GALNT10 antibody
See all GALNT10 primary antibodies
DescriptionRabbit polyclonal to GALNT10
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 16 amino acids near the N terminal of Human GALNT10 (NP_938080).
- Rat brain tissue lysate; Human brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106445 was purified by affinity chromatography via a peptide column.
Our Abpromise guarantee covers the use of ab106445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 69 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates.
Tissue specificityWidely expressed. Expressed at high level in small intestine, and at intermediate levels in stomach, pancreas, ovary, thyroid gland and spleen. Weakly expressed in other tissues.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain.
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- DKFZp586H0623 antibody
- FLJ00205 antibody
- FLJ11715 antibody
Immunofluorescence of GALNT10 in Human brain tissue with ab106445 at 20 ug/mL.
Immunohistochemistry of GALNT10 in Human brain tissue with ab106455 at 2.5 μg/mL.
Lane 1 : Anti-GALNT10 antibody (ab106445) at 1 µg/ml
Lane 2 : Anti-GALNT10 antibody (ab106445) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 69 kDa
ab106445 has not yet been referenced specifically in any publications.