Product nameAnti-GALNT2 antibody
See all GALNT2 primary antibodies
DescriptionRabbit polyclonal to GALNT2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HeLa cell lysate. IHC-P: Human pancreas tissue. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 65 kDa.|
FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region.
Tissue specificityWidely expressed.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain.
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
Cellular localizationGolgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists.
- Information by UniProt
- GalNAc T2 antibody
- GalNAc-T2 antibody
- Galnt2 antibody
All lanes : Anti-GALNT2 antibody (ab262868) at 0.4 µg/ml
Lane 1 : HeLa (transfected with control siRNA) cell lysate
Lanes 2-3 : HeLa (transfected with target-specific siRNA) cell lysate
Predicted band size: 65 kDa
Loading control: GAPDH.
PFA-fixed, Triton X-100 permeabilized human U-2 OS cells stained for GALNT2 (green) using ab262868 at 4 µg/ml in ICC/IF.
Paraffin-embedded human pancreas tissue stained for GALNT2 using ab262868 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262868 has not yet been referenced specifically in any publications.