Recombinant
Recombinant Anti-GALNT2 antibody [EPR9605(B)] (ab140637)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GALNT2 antibody [EPR9605(B)]
See all GALNT2 primary antibodies -
Description
Rabbit monoclonal [EPR9605(B)] to GALNT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GALNT2 aa 50-150. The exact sequence is proprietary.
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Positive control
- A549, HeLa, fetal heart, U87-MG and fetal liver cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9605(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab140637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
Domain
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists. - Information by UniProt
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Database links
- Entrez Gene: 2590 Human
- Entrez Gene: 108148 Mouse
- Entrez Gene: 292090 Rat
- Omim: 602274 Human
- SwissProt: Q10471 Human
- SwissProt: Q6PB93 Mouse
- Unigene: 654649 Human
- Unigene: 33808 Mouse
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Alternative names
- GalNAc T2 antibody
- GalNAc-T2 antibody
- Galnt2 antibody
see all
Images
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![Western blot - Anti-GALNT2 antibody [EPR9605(B)] (ab140637)](https://www.abcam.com/ps/products/140/ab140637/Images/ab140637-155960-anti-galnt2-antibody-epr9605b-western-blot.jpg)
Western blot - Anti-GALNT2 antibody [EPR9605(B)] (ab140637)All lanes : Anti-GALNT2 antibody [EPR9605(B)] (ab140637) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Protocols
Datasheets and documents
References
ab140637 has not yet been referenced specifically in any publications.
