Recombinant Anti-GALNT2 antibody [EPR9605(B)] (ab140637)
![Western blot - Anti-GALNT2 antibody [EPR9605(B)] (ab140637)](https://www.abcam.com/ps/products/140/ab140637/Images/ab140637-155960-anti-galnt2-antibody-epr9605b-western-blot.jpg "Western blot - Anti-GALNT2 antibody [EPR9605(B)] (ab140637)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9605(B)] to GALNT2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GALNT2 antibody [EPR9605(B)]
See all GALNT2 primary antibodies -
Description
Rabbit monoclonal [EPR9605(B)] to GALNT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GALNT2 aa 50-150. The exact sequence is proprietary.
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Positive control
- A549, HeLa, fetal heart, U87-MG and fetal liver cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9605(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab140637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
Target
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Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
Domain
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists. - Information by UniProt
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Database links
- Entrez Gene: 2590 Human
- Entrez Gene: 108148 Mouse
- Entrez Gene: 292090 Rat
- Omim: 602274 Human
- SwissProt: Q10471 Human
- SwissProt: Q6PB93 Mouse
- Unigene: 654649 Human
- Unigene: 33808 Mouse
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Alternative names
- GalNAc T2 antibody
- GalNAc-T2 antibody
- Galnt2 antibody
see all
Images
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Western blot - Anti-GALNT2 antibody [EPR9605(B)] (ab140637)All lanes : Anti-GALNT2 antibody [EPR9605(B)] (ab140637) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 65 kDa -
![Anti-GALNT2 antibody [EPR9605(B)] (ab140637)](https://www.abcam.com/ps/products/140/ab140637/Images/ab140637-2-benefits-of-recombinant-antibodies.png)
Anti-GALNT2 antibody [EPR9605(B)] (ab140637)
Datasheets and documents
References (0)
ab140637 has not yet been referenced specifically in any publications.
