Product nameAnti-GALNT2 antibody [EPR9605(B)]
See all GALNT2 primary antibodies
DescriptionRabbit monoclonal [EPR9605(B)] to GALNT2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GALNT2 aa 50-150. The exact sequence is proprietary.
- A549, HeLa, fetal heart, U87-MG and fetal liver cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab140637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 65 kDa.|
FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region.
Tissue specificityWidely expressed.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain.
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
Cellular localizationGolgi apparatus > Golgi stack membrane. Secreted. Resides preferentially in the trans and medial parts of the Golgi stack. A secreted form also exists.
- Information by UniProt
- GalNAc T2 antibody
- GalNAc-T2 antibody
- Galnt2 antibody
All lanes : Anti-GALNT2 antibody [EPR9605(B)] (ab140637) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 65 kDa
ab140637 has not yet been referenced specifically in any publications.