Overview

  • Product name

    Anti-GALNT6 antibody
  • Description

    Rabbit polyclonal to GALNT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 30-518 of Human GALNT6.

  • Positive control

    • 293T, A431, H1299 and MOLT4 cell lysates; HBL435 xenograft; A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.
  • Tissue specificity

    Expressed in placenta and trachea. Weakly expressed in brain and pancreas. Expressed in fibroblast. Weakly or not expressed in lung, liver, muscle, kidney, spleen, thymus, prostate, testis, ovary, intestine, colon, leukocyte, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland and bone marrow.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
    Contains 1 ricin B-type lectin domain.
  • Domain

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GALNAC T6 antibody
    • GalNAc transferase 6 antibody
    • GalNAc-T6 antibody
    • GalNAcT6 antibody
    • GALNT 6 antibody
    • GALNT6 antibody
    • GALT6_HUMAN antibody
    • Polypeptide GalNAc transferase 6 antibody
    • Polypeptide N acetylgalactosaminyltransferase 6 antibody
    • Polypeptide N-acetylgalactosaminyltransferase 6 antibody
    • pp GaNTase 6 antibody
    • pp-GaNTase 6 antibody
    • Protein UDP acetylgalactosaminyltransferase 6 antibody
    • Protein-UDP acetylgalactosaminyltransferase 6 antibody
    • UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 6 antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 6 (GalNAc T6) antibody
    • UDP N acetyl alpha D galactosamine:polypeptide N acetylgalactosaminyltransferase 6 antibody
    • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft labeling GALNT6 with ab151329 at 1/500 dilution.
  • Anti-GALNT6 antibody (ab151329) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 71 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling GALNT6 with ab151329 at 1/200 dilution. Right panel co-stained with Hoechst 33342.

References

ab151329 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab151329.
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