Anti-GALNT7 antibody (ab113743)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GALNT7 antibody
See all GALNT7 primary antibodies -
Description
Rabbit polyclonal to GALNT7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human GALNT7 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q80VA0 -
Positive control
- This antibody gave a positive signal in Mouse Colon Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 75 kDa). |
Target
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Function
Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified. -
Tissue specificity
Widely expressed. Expressed in uterus, retina, kidney, small intestine, omentum, stomach and CNS. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
Domain
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 51809 Human
- Entrez Gene: 108150 Mouse
- Entrez Gene: 100171466 Orangutan
- Entrez Gene: 29750 Rat
- Omim: 605005 Human
- SwissProt: Q86SF2 Human
- SwissProt: Q80VA0 Mouse
- SwissProt: Q9R0C5 Rat
see all -
Alternative names
- GalNAc T7 antibody
- GalNAc-T7 antibody
- GalNAcT7 antibody
see all
Images
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Western blot - Anti-GALNT7 antibody (ab113743)Anti-GALNT7 antibody (ab113743) at 1 µg/ml (Milk blocking (5%)) + Colon (Mouse) Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Additional bands at: 53 kDa (possible non-specific binding), 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab113743 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab113743 has not yet been referenced specifically in any publications.
