Anti-GALNT7 antibody (ab97645)
Key features and details
- Rabbit polyclonal to GALNT7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GALNT7 antibody
See all GALNT7 primary antibodies -
Description
Rabbit polyclonal to GALNT7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 24-485 of Human GALNT7 (NP_059119).
-
Positive control
- A431 whole cell lysate; 293T, H1299, HeLaS3, HepG2, Molt-4 and Raji cell lysates
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 75 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 75 kDa. |
Target
-
Function
Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified. -
Tissue specificity
Widely expressed. Expressed in uterus, retina, kidney, small intestine, omentum, stomach and CNS. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
Domain
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51809 Human
- Entrez Gene: 108150 Mouse
- Entrez Gene: 29750 Rat
- Omim: 605005 Human
- SwissProt: Q86SF2 Human
- SwissProt: Q80VA0 Mouse
- SwissProt: Q9R0C5 Rat
- Unigene: 548088 Human
see all -
Alternative names
- GalNAc T7 antibody
- GalNAc-T7 antibody
- GalNAcT7 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab97645 has been referenced in 3 publications.
- Cao Q et al. miR-125a-5p post-transcriptionally suppresses GALNT7 to inhibit proliferation and invasion in cervical cancer cells via the EGFR/PI3K/AKT pathway. Cancer Cell Int 20:117 (2020). PubMed: 32308562
- Möckl L et al. Quantitative Super-Resolution Microscopy of the Mammalian Glycocalyx. Dev Cell 50:57-72.e6 (2019). PubMed: 31105009
- Li Y et al. miR-30a-5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA. Mol Med Rep 13:4085-94 (2016). PubMed: 27035333