Key features and details
- Rabbit polyclonal to GALNT8
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GALNT8 antibody
DescriptionRabbit polyclonal to GALNT8
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human GALNT8 aa 502-629.
Database link: Q9NY28
- WB: RT4 and U251 MG cell lysates. ICC: U-2 OS cells. IHC-P: Human testis, rectum and cerebellum tissues.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionProbably catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.
Tissue specificityWidely expressed. Expressed in heart, skeletal muscle, kidney, liver, small intestine and placenta. Weakly expressed in colon, thymus, spleen, lung and leukocyte.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain.
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- GALNAC T8 antibody
- GalNAc-T8 antibody
- GALNT8 antibody
Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles. Antibody staining is shown in green.
All lanes : Anti-GALNT8 antibody (ab121374) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Developed using the ECL technique.
Immunohistochemical analysis of human testis tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
Immunohistochemical analysis of human rectum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
Immunohistochemical analysis of human cerebellum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
Immunohistochemical analysis of human liver tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
ab121374 has not yet been referenced specifically in any publications.