Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab59025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe activity of this receptor for the hormone galanin is mediated by G proteins of the Gi/Go family that inhibit adenylate cyclase activity. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the colon, small intestine and heart. ESTs have been isolated from nerve and fetal lung/testis/B cell libraries.
Cellular localizationCell membrane; Multi-pass membrane protein.
- gal receptor 1 antibody
- gal-1 receptor antibody
- GAL1R antibody
Anti-GALR1 antibody (ab59025) at 0.1 µg/ml + Human Heart lysate (35µg protein in RIPA buffer)
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
ab59025 has not yet been referenced specifically in any publications.