Key features and details
- Rabbit polyclonal to GALT
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GALT antibody
See all GALT primary antibodies
DescriptionRabbit polyclonal to GALT
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human GALT aa 1-379.
MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMK RPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFP ALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAV VDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQ REERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWP YQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGW HGAPTGSEAGANWNHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLT PEQAAERLRALPEVHYHLGQKDRETATIA
Database link: P07902
- WB: Mouse small intestine and kidney lysates; rat liver lysate. IP: HepG2 whole cell lysate. IHC-P: Human tonsil and adrenal gland tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 43 kDa.|
PathwayCarbohydrate metabolism; galactose metabolism.
Involvement in diseaseDefects in GALT are the cause of galactosemia (GALCT) [MIM:230400]. Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation.
Sequence similaritiesBelongs to the galactose-1-phosphate uridylyltransferase type 1 family.
- Information by UniProt
- Gal 1 P uridylyltransferase antibody
- Gal-1-P uridylyltransferase antibody
- Galactose 1 phosphate uridyl transferase antibody
Anti-GALT antibody (ab237805) at 1/500 dilution + Mouse small intestine lysate
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 43 kDa
All lanes : Anti-GALT antibody (ab237805) at 1/500 dilution
Lane 1 : Rat liver lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human tonsil tissue stained for GALT using ab237805 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for GALT using ab237805 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GALT (green) using ab237805 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
GALT was immunoprecipitated from 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab237805 at 1/200 dilution.
Lane 1: Control rabbit IgG IP in HepG2 whole cell lysate.
Lane 2: ab237805 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 μg (Input).
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237805 has not yet been referenced specifically in any publications.