Product nameAnti-GALT antibody [EPR12555]
See all GALT primary antibodies
DescriptionRabbit monoclonal [EPR12555] to GALT
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GALT aa 50-150. The exact sequence is proprietary.
Database link: P07902
- K562, MDA-MB-435 and HepG2 whole cell lysate (ab7900); Human fetal brain and fetal liver lysates; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 43 kDa.|
|IP||1/10 - 1/100.|
PathwayCarbohydrate metabolism; galactose metabolism.
Involvement in diseaseDefects in GALT are the cause of galactosemia (GALCT) [MIM:230400]. Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation.
Sequence similaritiesBelongs to the galactose-1-phosphate uridylyltransferase type 1 family.
- Information by UniProt
- Gal 1 P uridylyltransferase antibody
- Gal-1-P uridylyltransferase antibody
- Galactose 1 phosphate uridyl transferase antibody
Lanes 1, 3 and 5: GALT knockout HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: Wild-type HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab178406 observed at 43 kDa
Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 5 and 6: Merged (red and green) signal
ab178406 was shown to react with GALT when GALT knockout samples were used, along with additional cross-reactive bands. Wild-type and GALT knockout samples were subjected to SDS-PAGE. ab178406 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Human fetal liver lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This product has been referenced in:
- Tran H et al. Mast Cells Induce Blood Brain Barrier Damage in SCD by Causing Endoplasmic Reticulum Stress in the Endothelium. Front Cell Neurosci 13:56 (2019). Read more (PubMed: 30837844) »
- Sun YZ et al. Proteomic and bioinformatic analysis of condyloma acuminata: mild hyperthermia treatment reveals compromised HPV infectivity of keratinocytes via regulation of metabolism, differentiation and anti-viral responses. Int J Hyperthermia 36:383-393 (2019). Read more (PubMed: 30909744) »