Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350)

Mouse monoclonal gamma H2A.X (phospho S139) antibody [9F3]. Validated in WB, IP, IHC, Flow Cyt, ISH, ICC/IF and tested in Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human and more.

Publishing research using ab26350? Please let us know so that we can cite the reference in this datasheet.

ab26350 has been referenced in 78 publications.

  • Gao S  et al. LncRNA LCPAT1 is involved in DNA damage induced by CSE. Biochem Biophys Res Commun 508:512-515 (2019). PubMed: 30509493
  • Zhao H  et al. Single-Cell Transcriptomics of Human Oocytes: Environment-Driven Metabolic Competition and Compensatory Mechanisms During Oocyte Maturation. Antioxid Redox Signal 30:542-559 (2019). PubMed: 29486586
  • Plappert-Helbig U  et al. Gamma-H2AX immunofluorescence for the detection of tissue-specific genotoxicity in vivo. Environ Mol Mutagen 60:4-16 (2019). PubMed: 30307065
  • Zhang HN  et al. MGMT is down-regulated independently of promoter DNA methylation in rats with all-trans retinoic acid-induced spina bifida aperta. Neural Regen Res 14:361-368 (2019). PubMed: 30531021
  • Li J & Zheng J Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in vitro. Mol Med Rep 19:821-830 (2019). PubMed: 30569095
  • Wu Q  et al. Cascade enzymes within self-assembled hybrid nanogel mimicked neutrophil lysosomes for singlet oxygen elevated cancer therapy. Nat Commun 10:240 (2019). PubMed: 30651559
  • Was H  et al. Histone deacetylase inhibitors exert anti-tumor effects on human adherent and stem-like glioma cells. Clin Epigenetics 11:11 (2019). PubMed: 30654849
  • Piras AR  et al. Structure of preantral follicles, oxidative status and developmental competence of in vitro matured oocytes after ovary storage at 4 °C in the domestic cat model. Reprod Biol Endocrinol 16:76 (2018). PubMed: 30097048
  • Latorre E  et al. Mitochondria-targeted hydrogen sulfide attenuates endothelial senescence by selective induction of splicing factors HNRNPD and SRSF2. Aging (Albany NY) 10:1666-1681 (2018). PubMed: 30026406
  • Li HX  et al. Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study. Oncol Rep 39:1609-1618 (2018). PubMed: 29436690
  • Prevc A  et al. Electrochemotherapy with cisplatin or bleomycin in head and neck squamous cell carcinoma: Improved effectiveness of cisplatin in HPV-positive tumors. Bioelectrochemistry 123:248-254 (2018). ICC/IF . PubMed: 29898433
  • Caridi CP  et al. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature 559:54-60 (2018). PubMed: 29925946
  • Zhao X  et al. HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma. Cell Death Dis 9:61 (2018). WB ; Human . PubMed: 29352124
  • Cai B  et al. MiR-16-5p targets SESN1 to regulate the p53 signaling pathway, affecting myoblast proliferation and apoptosis, and is involved in myoblast differentiation. Cell Death Dis 9:367 (2018). PubMed: 29511169
  • Tripathi V  et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). PubMed: 29523790
  • York D  et al. Enrofloxacin enhances the effects of chemotherapy in canine osteosarcoma cells with mutant and wild-type p53. Vet Comp Oncol 15:1087-1100 (2017). PubMed: 27333821
  • Yan S  et al. Sunitinib induces genomic instability of renal carcinoma cells through affecting the interaction of LC3-II and PARP-1. Cell Death Dis 8:e2988 (2017). PubMed: 28796254
  • Liu JC  et al. Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro. Cell Death Dis 8:e2966 (2017). PubMed: 28771232
  • Espana-Agusti J  et al. Loss of PBRM1 rescues VHL dependent replication stress to promote renal carcinogenesis. Nat Commun 8:2026 (2017). ICC ; Mouse . PubMed: 29229903
  • Guo R  et al. Physiological oxygen tension reduces hepatocyte dedifferentiation in in vitro culture. Sci Rep 7:5923 (2017). PubMed: 28724942
  • Yu C  et al. Critical Role of Hepatic Cyp450s in the Testis-Specific Toxicity of (5R)-5-Hydroxytriptolide in C57BL/6 Mice. Front Pharmacol 8:832 (2017). IHC-P . PubMed: 29209210
  • Cao Z  et al. Maternal histone acetyltransferase KAT8 is required for porcine preimplantation embryo development. Oncotarget 8:90250-90261 (2017). PubMed: 29163825
  • Zhang XF  et al. Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3). Int J Nanomedicine 12:7551-7575 (2017). PubMed: 29075115
  • Detappe A  et al. Ultrasmall Silica-Based Bismuth Gadolinium Nanoparticles for Dual Magnetic Resonance-Computed Tomography Image Guided Radiation Therapy. Nano Lett 17:1733-1740 (2017). PubMed: 28145723
  • Kondrashova O  et al. Secondary Somatic Mutations RestoringRAD51CandRAD51DAssociated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma. Cancer Discov 7:984-998 (2017). PubMed: 28588062
  • Jin ZL & Kim NH RAD51 maintains chromosome integrity and mitochondrial distribution during porcine oocyte maturation in vitro. J Reprod Dev 63:489-496 (2017). PubMed: 28757527
  • Bai DP  et al. Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells. Int J Nanomedicine 12:6521-6535 (2017). PubMed: 28919752
  • Boege Y  et al. A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development. Cancer Cell 32:342-359.e10 (2017). PubMed: 28898696
  • Zhang C  et al. Radioresistance of chordoma cells is associated with the ATM/ATR pathway, in which RAD51 serves as an important downstream effector. Exp Ther Med 14:2171-2179 (2017). PubMed: 28962138
  • Li J  et al. Targeting Plk1 to Enhance Efficacy of Olaparib in Castration-Resistant Prostate Cancer. Mol Cancer Ther 16:469-479 (2017). PubMed: 28069876
  • Xiang Y  et al. RNA m6A methylation regulates the ultraviolet-induced DNA damage response. Nature 543:573-576 (2017). PubMed: 28297716
  • Zhang H  et al. CAF-secreted CXCL1 conferred radioresistance by regulating DNA damage response in a ROS-dependent manner in esophageal squamous cell carcinoma. Cell Death Dis 8:e2790 (2017). PubMed: 28518141
  • Kong F  et al. Differential regulation of spermatogenic process by Lkb1 isoforms in mouse testis. Cell Death Dis 8:e3121 (2017). IHC-P ; Mouse . PubMed: 29022902
  • Chandran JS  et al. Site Specific Modification of Adeno-Associated Virus Enables Both Fluorescent Imaging of Viral Particles and Characterization of the Capsid Interactome. Sci Rep 7:14766 (2017). PubMed: 29116194
  • Song X  et al. Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo. Redox Biol 11:219-230 (2017). ICC/IF ; Human . PubMed: 28012437
  • Xie Q  et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res 45:2472-2489 (2017). PubMed: 27924000
  • ElMokh O  et al. Combined MEK and Pi3'-kinase inhibition reveals synergy in targeting thyroid cancer in vitro and in vivo. Oncotarget 8:24604-24620 (2017). WB ; Mouse . PubMed: 28445948
  • Laugeray A  et al. Perinatal Exposure to the Cyanotoxin ß-N-Méthylamino-L-Alanine (BMAA) Results in Long-Lasting Behavioral Changes in Offspring-Potential Involvement of DNA Damage and Oxidative Stress. Neurotox Res N/A:N/A (2017). IF ; Mouse . PubMed: 28879461
  • Studencka M & Schaber J Senoptosis: non-lethal DNA cleavage as a route to deep senescence. Oncotarget 8:30656-30671 (2017). WB, IF . PubMed: 28427150
  • Monteforte R  et al. SNEV(Prp19/PSO4) deficiency increases PUVA-induced senescence in mouse skin. Exp Dermatol 25:212-7 (2016). WB . PubMed: 26663487
  • Much C  et al. Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein. PLoS Genet 12:e1006095 (2016). PubMed: 27254021
  • Suetens A  et al. Higher Initial DNA Damage and Persistent Cell Cycle Arrest after Carbon Ion Irradiation Compared to X-irradiation in Prostate and Colon Cancer Cells. Front Oncol 6:87 (2016). ICC . PubMed: 27148479
  • Zhang X  et al. Solar Simulated Ultraviolet Radiation Induces Global Histone Hypoacetylation in Human Keratinocytes. PLoS One 11:e0150175 (2016). IF . PubMed: 26918332
  • Zeller A  et al. A proposal for a novel rationale for critical effect size in dose-response analysis based on a multi-endpoint in vivo study with methyl methanesulfonate. Mutagenesis 31:239-53 (2016). IHC ; Rat . PubMed: 26590612
  • McAndrew EN  et al. The synthetic lethal killing of RAD54B-deficient colorectal cancer cells by PARP1 inhibition is enhanced with SOD1 inhibition. Oncotarget 7:87417-87430 (2016). PubMed: 27902462
  • Hu MH  et al. Specific targeting of telomeric multimeric G-quadruplexes by a new triaryl-substituted imidazole. Nucleic Acids Res N/A:N/A (2016). ICC/IF ; Human . PubMed: 27923993
  • Yang Q  et al. NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis. Cell Death Differ 23:1406-16 (2016). PubMed: 27035619
  • Hou F  et al. Ameliorative Effect of Grape Seed Proanthocyanidin Extract on Cadmium-Induced Meiosis Inhibition During Oogenesis in Chicken Embryos. Anat Rec (Hoboken) 299:450-60 (2016). PubMed: 26799944
  • Fischer U  et al. Gene amplification during myogenic differentiation. Oncotarget 7:6864-77 (2016). PubMed: 26760505
  • Boysen G  et al. SPOP mutation leads to genomic instability in prostate cancer. Elife 4:N/A (2015). ICC/IF ; Mouse . PubMed: 26374986
  • Bunch H  et al. Transcriptional elongation requires DNA break-induced signalling. Nat Commun 6:10191 (2015). ICC/IF ; Human . PubMed: 26671524
  • Tarrade S  et al. Histone H2AX Is Involved in FoxO3a-Mediated Transcriptional Responses to Ionizing Radiation to Maintain Genome Stability. Int J Mol Sci 16:29996-30014 (2015). WB ; Mouse . PubMed: 26694365
  • Xu B  et al. Oridonin inhibits BxPC-3 cell growth through cell apoptosis. Acta Biochim Biophys Sin (Shanghai) 47:164-73 (2015). WB ; Human . PubMed: 25651847
  • Nadeau MÈ  et al. Pharmacological targeting of valosin containing protein (VCP) induces DNA damage and selectively kills canine lymphoma cells. BMC Cancer 15:479 (2015). WB ; Dog . PubMed: 26104798
  • DeLoughery Z  et al. DNA double-strand breaks by Cr(VI) are targeted to euchromatin and cause ATR-dependent phosphorylation of histone H2AX and its ubiquitination. Toxicol Sci 143:54-63 (2015). PubMed: 25288669
  • Mohan M  et al. Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques. J Immunol 194:291-306 (2015). PubMed: 25452565
  • Jørgensen A  et al. Ex vivo culture of human fetal gonads: manipulation of meiosis signalling by retinoic acid treatment disrupts testis development. Hum Reprod 30:2351-63 (2015). PubMed: 26251460
  • Fan G  et al. Loss of KLF14 triggers centrosome amplification and tumorigenesis. Nat Commun 6:8450 (2015). PubMed: 26439168
  • Yang KS  et al. Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Sci Rep 5:10129 (2015). PubMed: 25984718
  • Lapytsko A  et al. FoCo: a simple and robust quantification algorithm of nuclear foci. BMC Bioinformatics 16:392 (2015). ICC/IF ; Human . PubMed: 26589438
  • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). PubMed: 26212453
  • Rastgou Talemi S  et al. Development of a robust DNA damage model including persistent telomere-associated damage with application to secondary cancer risk assessment. Sci Rep 5:13540 (2015). IF . PubMed: 26359627
  • Nie P  et al. Synergistic Induction of Erlotinib-Mediated Apoptosis by Resveratrol in Human Non-Small-Cell Lung Cancer Cells by Down-Regulating Survivin and Up-Regulating PUMA. Cell Physiol Biochem 35:2255-71 (2015). PubMed: 25895606
  • Kong Q  et al. Identification and characterization of an oocyte factor required for porcine nuclear reprogramming. J Biol Chem 289:6960-8 (2014). PubMed: 24474691
  • Deng S  et al. Dexrazoxane may prevent doxorubicin-induced DNA damage via depleting both topoisomerase II isoforms. BMC Cancer 14:842 (2014). PubMed: 25406834
  • Chao OS & Goodman OB Synergistic loss of prostate cancer cell viability by coinhibition of HDAC and PARP. Mol Cancer Res 12:1755-66 (2014). PubMed: 25127709
  • Sutherland JM  et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod 90:92 (2014). ICC/IF ; Mouse . PubMed: 24671879
  • Di Giacomo M  et al. G9a co-suppresses LINE1 elements in spermatogonia. Epigenetics Chromatin 7:24 (2014). PubMed: 25276231
  • Lowe D & Raj K Premature aging induced by radiation exhibits pro-atherosclerotic effects mediated by epigenetic activation of CD44 expression. Aging Cell 13:900-10 (2014). IF ; Human . PubMed: 25059316
  • Xiao L  et al. The lipophilic vitamin C derivative, 6-o-palmitoylascorbate, protects human lymphocytes, preferentially over ascorbate, against X-ray-induced DNA damage, lipid peroxidation, and protein carbonylation. Mol Cell Biochem 394:247-59 (2014). PubMed: 24898782
  • Flassig RJ  et al. Experimental design, validation and computational modeling uncover DNA damage sensing by DNA-PK and ATM. Mol Biosyst 10:1978-86 (2014). PubMed: 24833308
  • Kracikova M  et al. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ 20:576-88 (2013). PubMed: 23306555
  • Triscott J  et al. Personalizing the treatment of pediatric medulloblastoma: Polo-like kinase 1 as a molecular target in high-risk children. Cancer Res 73:6734-44 (2013). PubMed: 24019381
  • Kefala M  et al. Increased expression of phosphorylated NBS1, a key molecule of the DNA damage response machinery, is an adverse prognostic factor in patients with de novo myelodysplastic syndromes. Leuk Res 37:1576-82 (2013). Human . PubMed: 24054861
  • Di Giacomo M  et al. Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis. Mol Cell 50:601-8 (2013). PubMed: 23706823
  • Matveevsky SN  et al. Synaptonemal complex analysis of interracial hybrids between the Moscow and Neroosa chromosomal races of the common shrew Sorex araneus showing regular formation of a complex meiotic configuration (ring-of-four). Comp Cytogenet 6:301-14 (2012). PubMed: 24260670
  • Pinton A  et al. Influence of sex on the meiotic segregation of a t(13;17) Robertsonian translocation: a case study in the pig. Hum Reprod 24:2034-43 (2009). In situ hybridization ; Pig . PubMed: 19403564
  • Pinton A  et al. Meiotic studies in an azoospermic boar carrying a Y;14 translocation. Cytogenet Genome Res 120:106-11 (2008). ICC/IF ; Pig . PubMed: 18467832

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