Overview

  • Product name

    Anti-gamma Actin antibody [2A3]
    See all gamma Actin primary antibodies
  • Description

    Mouse monoclonal [2A3] to gamma Actin
  • Host species

    Mouse
  • Specificity

    This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.

    This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).

  • Tested applications

    Suitable for: IHC-P, WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig, Zebrafish
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide (Ac-

    EEEIAALVI

    -COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin

  • Positive control

    • ICC/IF: Human dermal fibroblast cells. IHC-P: Human small intestine tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2A3
  • Myeloma

    NS0
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/500.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Images

  • Human dermal fibroblasts stained for gamma Actin (green) using ab123034 at 1/500 dilution in ICC/IF.

  • Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml

References

This product has been referenced in:

  • Yan Y  et al. RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma. Onco Targets Ther 12:1691-1703 (2019). Read more (PubMed: 30881024) »
  • Pan L  et al. miR-125a induces apoptosis, metabolism disorder and migrationimpairment in pancreatic cancer cells by targeting Mfn2-related mitochondrial fission. Int J Oncol 53:124-136 (2018). Read more (PubMed: 29749475) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 27 2017

Answer

Yes , this antibody does not react with other isoforms and it binds to actin gamma 2 only.

Read More

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