Overview

  • Product name

  • Description

    Rabbit polyclonal to gamma Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within gamma Actin aa 188-215 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    YLMKILTERGYSFTTTAEREIVRDIKEK


    Database link: P63261

  • Positive control

    • K562 and HepG2 cell line lysates; 293 cell lysate transiently transfected with gamma Actin; Human Spleen tissue; HepG2 cells; K562 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/200.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Images

  • All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution

    Lane 1 : K562 cell line lysate
    Lane 2 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa

  • All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution

    Lane 1 : 293 cell lysate non-transfected
    Lane 2 : 293 cell lysate transiently transfected with gamma Actin

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 42 kDa

  • Flow cytometry of K562 cells using ab194952 at 1/10 (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of  HepG2 cells labeling gamma Actin with ab194952 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling gamma Actin with ab194952 at 1/100.

References

This product has been referenced in:

  • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). WB . Read more (PubMed: 27860183) »
  • Zhang Y  et al. Cryptotanshinone targets tumor-initiating cells through down-regulation of stemness genes expression. Oncol Lett 11:3803-3812 (2016). Read more (PubMed: 27313698) »
See all 4 Publications for this product

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