Key features and details
- Rabbit polyclonal to gamma Actin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-gamma Actin antibody
See all gamma Actin primary antibodies
DescriptionRabbit polyclonal to gamma Actin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
Recombinant fragment corresponding to Human gamma Actin aa 140-361. (BC012050).
LSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYL MKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS YELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDI RKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSV WIGGSILASLSTFQQMWISKQE
Database link: P63261
- A549, HeLa and NIH3T3 cell lysates; Human fetal muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab199406 in the following tested applications.
|IHC-P||1/100 - 1/500.|
|WB||1/200 - 1/1000. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the actin family.
modificationsThe methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ACT antibody
- ACTB antibody
- ACTG antibody
All lanes : Anti-gamma Actin antibody (ab199406) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Predicted band size: 42 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal muscle tissue labeling gamma Actin with ab199406 at 1/100 dilution.
ab199406 has been referenced in 1 publication.
- Qiao Y et al. Oxidized-low density lipoprotein accumulates cholesterol esters via the PKCa-adipophilin-ACAT1 pathway in RAW264.7 cells. Mol Med Rep 12:3599-3606 (2015). PubMed: 26017812