Key features and details
- Rabbit polyclonal to gamma Actin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-gamma Actin antibody
See all gamma Actin primary antibodies
DescriptionRabbit polyclonal to gamma Actin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human gamma Actin. A peptide corresponding to residues surrounding amino acid 365 of Human gamma Actin.
Database link: NM_001614
- WB: Human SW-620 cell lysates. IHC-P: Human fetal muscle tissue.
FormLyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||1/100 - 1/500.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the actin family.
modificationsThe methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ACT antibody
- ACTB antibody
- ACTG antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal muscle tissue labelling gamma Actin with ab84479 at a dilution of 1/100.
Anti-gamma Actin antibody (ab84479) at 1/300 dilution + Human SW-620 cell lysates
Predicted band size: 42 kDa
Observed band size: 42 kDa
ab84479 has not yet been referenced specifically in any publications.