Overview

  • Product name

  • Description

    Rabbit polyclonal to gamma Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human gamma Actin. A peptide corresponding to residues surrounding amino acid 365 of Human gamma Actin.
    Database link: NM_001614

  • Positive control

    • WB: Human SW-620 cell lysates. IHC-P: Human fetal muscle tissue.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal muscle tissue labelling gamma Actin with ab84479 at a dilution of 1/100.

  • Anti-gamma Actin antibody (ab84479) at 1/300 dilution + Human SW-620 cell lysates

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

References

ab84479 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84479.
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