Recombinant Anti-gamma Actin antibody [EPR19353-2] (ab200046)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19353-2] to gamma Actin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-gamma Actin antibody [EPR19353-2]
See all gamma Actin primary antibodies -
Description
Rabbit monoclonal [EPR19353-2] to gamma Actin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human Gamma actin (ACTG1) recombinant protein; Jurkat, A549, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, fetal kidney and fetal brain lysates; Mouse kidney and spleen lysates; Rat kidney, spleen and brain lysates. IP: HeLa whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19353-2 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
|
IP |
1/30.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
IP
1/30. |
Target
-
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsThe methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 71 Human
- Entrez Gene: 11465 Mouse
- Entrez Gene: 100361457 Rat
- Entrez Gene: 287876 Rat
- Omim: 102560 Human
- SwissProt: P63261 Human
- SwissProt: P63260 Mouse
- SwissProt: P63259 Rat
see all -
Alternative names
- ACT antibody
- ACTB antibody
- ACTG antibody
see all
Images
-
All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution
Lane 1 : Human Gamma actin (ACTG1) recombinant protein at 0.01 µg
Lane 2 : Human Beta actin (ACTB) recombinant protein at 0.02 µg
Lane 3 : Human Gamma-2 actin(ACTG2) recombinant protein at 0.02 µg
Lane 4 : Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg
Lane 5 : Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg
Lane 6 : Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Human Gamma actin (ACTG1) contains aa2-182 with His-tag.
Human Beta actin (ACTB) contains aa1-100 with GST and His tag.
Human Gamma-2 actin(ACTG2) contains aa1-200 with His-tag.
Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag.
Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag.
Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag
-
All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/3: 3 minutes; Lane 2: 30 seconds.
-
All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 30 seconds; Lane 4/5: 3 minutes; Lane 6-8: 1 minute
-
gamma Actin was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab200046 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200046 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 μg (Input).
Lane 2: ab200046 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200046 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (4)
ab200046 has been referenced in 4 publications.
- Tang Y et al. Actin gamma 1 is a critical regulator of pancreatic ductal adenocarcinoma. Saudi J Gastroenterol 28:239-246 (2022). PubMed: 34856725
- Li H et al. Mst1 deletion attenuates renal ischaemia-reperfusion injury: The role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3ß-p53 signalling pathway. Redox Biol 20:261-274 (2019). PubMed: 30384260
- Qin R et al. Sirt1 inhibits HG-induced endothelial injury: Role of Mff-based mitochondrial fission and F-actin homeostasis-mediated cellular migration. Int J Mol Med 44:89-102 (2019). PubMed: 31115479
- Zhou H et al. Empagliflozin rescues diabetic myocardial microvascular injury via AMPK-mediated inhibition of mitochondrial fission. Redox Biol 15:335-346 (2017). PubMed: 29306791