Recombinant Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
![Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)](https://www.abcam.com/ps/products/223/ab223147/Images/ab223147-320811-anti-gamma-actin-antibody-epr19353-2-immunoprecipitation.jpg "Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19353-2] to gamma Actin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free
See all gamma Actin primary antibodies -
Description
Rabbit monoclonal [EPR19353-2] to gamma Actin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment aa 1-200. The exact sequence is proprietary.
Database link: P63261 -
Positive control
- WB: Human Gamma actin (ACTG1) recombinant protein; Jurkat, A549, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, fetal kidney and fetal brain lysates; Mouse kidney and spleen lysates; Rat kidney, spleen and brain lysates. IP: HeLa whole cell lysate.
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General notes
Ab223147 is the carrier-free version of ab200046. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab223147 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19353-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab223147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsThe methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 71 Human
- Entrez Gene: 11465 Mouse
- Entrez Gene: 100361457 Rat
- Entrez Gene: 287876 Rat
- Omim: 102560 Human
- SwissProt: P63261 Human
- SwissProt: P63260 Mouse
- SwissProt: P63259 Rat
see all -
Alternative names
- ACT antibody
- ACTB antibody
- ACTG antibody
see all
Images
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Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
gamma Actin was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab200046 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200046 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 μg (Input).
Lane 2: ab200046 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200046 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200046).
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![Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)](https://www.abcam.com/ps/products/223/ab223147/Images/ab223147-2-benefits-of-recombinant-antibodies.png)
Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab223147 has not yet been referenced specifically in any publications.
