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RecombinantRabMAb

Recombinant Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)

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  • Certificate of Compliance
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Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
  • Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19353-2] to gamma Actin - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human gamma Actin protein (ab157841)

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Overview

  • Product name

    Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free
    See all gamma Actin primary antibodies
  • Description

    Rabbit monoclonal [EPR19353-2] to gamma Actin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment aa 1-200. The exact sequence is proprietary.
    Database link: P63261

  • Positive control

    • WB: Human Gamma actin (ACTG1) recombinant protein; Jurkat, A549, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, fetal kidney and fetal brain lysates; Mouse kidney and spleen lysates; Rat kidney, spleen and brain lysates. IP: HeLa whole cell lysate.
  • General notes

    Ab223147 is the carrier-free version of ab200046. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223147 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19353-2
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Alternative Versions

    • Anti-gamma Actin antibody [EPR19353-2] (ab200046)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human gamma Actin protein (ab157841)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

Our Abpromise guarantee covers the use of ab223147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTG1 are the cause of deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    The methylhistidine determined by Bienvenut et al is assumed to be the tele-methylhistidine isomer by similarity to the mouse ortholog.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P63261 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 71 Human
    • Entrez Gene: 11465 Mouse
    • Entrez Gene: 100361457 Rat
    • Entrez Gene: 287876 Rat
    • Omim: 102560 Human
    • SwissProt: P63261 Human
    • SwissProt: P63260 Mouse
    • SwissProt: P63259 Rat
    • Unigene: 514581 Human
    • Unigene: 196173 Mouse
    • Unigene: 426706 Mouse
    • Unigene: 101464 Rat
    • Unigene: 106826 Rat
    see all
  • Alternative names

    • ACT antibody
    • ACTB antibody
    • ACTG antibody
    • ACTG_HUMAN antibody
    • actg1 antibody
    • Actin antibody
    • Actin, cytoplasmic 2 antibody
    • Actin, gamma 1 antibody
    • Actin, gamma 1 propeptide antibody
    • Actin, gamma antibody
    • BRWS2 antibody
    • cytoplasmic 2 antibody
    • Cytoskeletal gamma actin antibody
    • Deafness, autosomal dominant 20 antibody
    • Deafness, autosomal dominant 26 antibody
    • DFNA20 antibody
    • DFNA26 antibody
    • epididymis luminal protein 176 antibody
    • Gamma-actin antibody
    • HEL-176 antibody
    • N-terminally processed antibody
    see all

Images

  • Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
    Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)

    gamma Actin was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab200046 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200046 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10 μg (Input).

    Lane 2: ab200046 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200046 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200046).

  • Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)
    Anti-gamma Actin antibody [EPR19353-2] - BSA and Azide free (ab223147)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab223147? Please let us know so that we can cite the reference in this datasheet.

    ab223147 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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