Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9875(B)] to gamma Adaptin
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-gamma Adaptin antibody [EPR9875(B)]
See all gamma Adaptin primary antibodies
DescriptionRabbit monoclonal [EPR9875(B)] to gamma Adaptin
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human gamma Adaptin (C terminal). The exact sequence is proprietary.
- HeLa and 293T cell lysates. Human lung carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 91 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSubunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the adaptor complexes large subunit family.
Contains 1 GAE domain.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
- Information by UniProt
- Adapter-related protein complex 1 subunit gamma-1 antibody
- Adaptor protein complex AP 1 gamma 1 subunit antibody
- Adaptor protein complex AP 1 subunit gamma 1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: gamma Adaptin knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human fetal brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab167153 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167153 was shown to specifically react with gamma Adaptin when gamma Adaptin knockout samples were used. Wild-type and gamma Adaptin knockout samples were subjected to SDS-PAGE. ab167153 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling gamma Adaptin with ab167153 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 91 kDa
ab167153 has been referenced in 1 publication.
- Decara J et al. The adiponectin promoter activator NP-1 induces high levels of circulating TNFa and weight loss in obese (fa/fa) Zucker rats. Sci Rep 8:9858 (2018). PubMed: 29959379