Overview

  • Product name
    Anti-gamma Adaptin antibody [EPR9875(B)]
    See all gamma Adaptin primary antibodies
  • Description
    Rabbit monoclonal [EPR9875(B)] to gamma Adaptin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human gamma Adaptin (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa and 293T cell lysates. Human lung carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9875(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 91 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the adaptor complexes large subunit family.
      Contains 1 GAE domain.
    • Cellular localization
      Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter-related protein complex 1 subunit gamma-1 antibody
      • Adaptor protein complex AP 1 gamma 1 subunit antibody
      • Adaptor protein complex AP 1 subunit gamma 1 antibody
      • Adaptor protein complex AP-1 subunit gamma-1 antibody
      • Adaptor related protein complex 1 gamma 1 subunit antibody
      • ADTG antibody
      • AP 1 complex subunit gamma 1 antibody
      • AP-1 complex subunit gamma-1 antibody
      • AP1G1 antibody
      • AP1G1_HUMAN antibody
      • CLAPG1 antibody
      • Clathrin assembly protein complex 1 gamma 1 large chain antibody
      • Clathrin assembly protein complex 1 gamma large chain antibody
      • Clathrin assembly protein complex 1 gamma-1 large chain antibody
      • Clathrin associated/assembly/adaptor protein large gamma 1 antibody
      • Gamma1 adaptin antibody
      • Gamma1-adaptin antibody
      • Golgi adaptor HA1/AP1 adaptin gamma subunit antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma 1 antibody
      • Golgi adaptor HA1/AP1 adaptin subunit gamma-1 antibody
      • MGC18255 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: gamma Adaptin knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab167153 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab167153 was shown to specifically react with gamma Adaptin when gamma Adaptin knockout samples were used. Wild-type and gamma Adaptin knockout samples were subjected to SDS-PAGE. ab167153 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling gamma Adaptin with ab167153 at 1/50 dilution.
    • All lanes : Anti-gamma Adaptin antibody [EPR9875(B)] (ab167153) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 91 kDa

    References

    This product has been referenced in:
    • Decara J  et al. The adiponectin promoter activator NP-1 induces high levels of circulating TNFa and weight loss in obese (fa/fa) Zucker rats. Sci Rep 8:9858 (2018). Read more (PubMed: 29959379) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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